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<p><i>Lactobacillus acidophillus</i> LA-832 and <i>Bifidobacterium longum</i> BL-101 were chosen as potential probiotics. These strains were encapsulated by sodium alginate and chitosan as wall materials. Five bead formulations were made for each strain E<sub>0</sub> (100% chitosan), E<sub>1</sub> (75% chitosan + 25% sodium alginate), E<sub>2</sub> (50% chitosan + 50% sodium alginate), E<sub>3</sub> (25% chitosan + 75% sodium alginate), E<sub>4</sub> (100% sodium alginate) and encapsulated by extrusion. The survivability of encapsulated <i>L. acidophilus</i> LA-832 and <i>B. longum</i> BL-101 were determined through their resistance to Simulated Gastric Juice (SGJ), tolerance to bile salt, and release profile, storage stability after 21&#xa0;days at 4&#xa0;°C. These encapsulated strains showed significant (<i>p</i> &lt; 0.05) tolerance to bile salts and resistance to gastric juice. E<sub>3</sub>, E<sub>4</sub> beads showed greater resistance to SGJ (at pH 2) and bile salts solution (2%) but significantly slower release. Encapsulation efficiency, size, yield and textural properties of beads remarkably increased with the increase in concentration of sodium alginate and chitosan. The scanning electron microscopy revealed that alginate beads have various pores but addition of chitosan improved the structural properties of beads. FTIR results confirmed the presence of sodium alginate and chitosan in respective treatments. Strains were subjected to efficacy trial after encapsulation. The findings showed that alginate-chitosan beads assist as a potential carrier for long-term survival and preservation of probiotics. Moreover, <i>L. acidophilus</i> and <i>B. longum</i> may further encapsulated by using spray drying, emulsion, spray chilling, freeze drying, etc. To effectively distribute probiotics, probiotic yoghurt and other milk-based products should be developed.</p>

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Microencapsulation of Lactobacillus acidophilus LA-832 and Bifidobacterium longum BL-101 by using sodium alginate and chitosan to improve the survival in simulated gastrointestinal conditions

  • 期刊论文

  • 发表日期：

    2025年6月5日

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  • Fiza Sana Ullah,
  • Muhammad Saeed,
  • Muhammad Asim Shabbir,
  • Beenish Israr

摘要

Lactobacillus acidophillus LA-832 and Bifidobacterium longum BL-101 were chosen as potential probiotics. These strains were encapsulated by sodium alginate and chitosan as wall materials. Five bead formulations were made for each strain E0 (100% chitosan), E1 (75% chitosan + 25% sodium alginate), E2 (50% chitosan + 50% sodium alginate), E3 (25% chitosan + 75% sodium alginate), E4 (100% sodium alginate) and encapsulated by extrusion. The survivability of encapsulated L. acidophilus LA-832 and B. longum BL-101 were determined through their resistance to Simulated Gastric Juice (SGJ), tolerance to bile salt, and release profile, storage stability after 21 days at 4 °C. These encapsulated strains showed significant (p < 0.05) tolerance to bile salts and resistance to gastric juice. E3, E4 beads showed greater resistance to SGJ (at pH 2) and bile salts solution (2%) but significantly slower release. Encapsulation efficiency, size, yield and textural properties of beads remarkably increased with the increase in concentration of sodium alginate and chitosan. The scanning electron microscopy revealed that alginate beads have various pores but addition of chitosan improved the structural properties of beads. FTIR results confirmed the presence of sodium alginate and chitosan in respective treatments. Strains were subjected to efficacy trial after encapsulation. The findings showed that alginate-chitosan beads assist as a potential carrier for long-term survival and preservation of probiotics. Moreover, L. acidophilus and B. longum may further encapsulated by using spray drying, emulsion, spray chilling, freeze drying, etc. To effectively distribute probiotics, probiotic yoghurt and other milk-based products should be developed.