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<p>The present work reports the secretion of biosurfactant by probiotic <i>Lactobacillus plantarum</i> SVP2 (SVP2-BS) in a previously formulated fermented EPS rich milk whey beverage. The cell-free BS-SVP2 was extracted from the fermented milk whey beverage by acid precipitation giving yield of 9.62&#xa0;g/L. The surface of SVP2-BS was fibrous network-like with presence of elemental carbon, oxygen and nitrogen in high amounts, as analysed by SEM-EDAX. SVP2-BS was confirmed as a lipoprotein by quantitative analysis and FT-IR characterization which revealed the presence of N-H, C = C, C-H<sub>2</sub>, C-O-C, C-O and C-H functional groups. The biofilm development of <i>P. aeruginosa</i> and <i>E. coli</i> was hindered by 96 ± 1.92% and 57 ± 1.14% on Ti-6Al-4&#xa0;V alloy plates treated with 1.0&#xa0;g/L SVP2-BS in comparison with untreated control plates. Additionally, SVP2-BS exhibited anti-cancer effects against HCT 116 colorectal cell lines (IC<sub>50</sub>:20&#xa0;µg/mL), whereas exposure at 1000&#xa0;µg/mL on IEC 6 mouse non-tumor epithelial cell lines, a viability of 60.2% was retained. The novelty of this study is that it reports the production of a bio-active biosurfactant with potent anti-biofilm and anti-cancer activity in a probiotic fermented bioactive EPS rich milk why based beverage, offering the consumer triple benefits of probiotic <i>L. plantarum</i> SVP2, its bioactive EPS and biosurfactant.</p>

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Anti-biofilm and anti-cancer effects of biosurfactant from Lactobacillus plantarum SVP2 in fermented milk whey beverage

  • 期刊论文

  • 发表日期：

    2025年5月15日

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  • Louella Concepta Goveas,
  • Pavankumar Khanapur,
  • D. M. Chetan,
  • Keerti Nannuri,
  • R. K. Shishir,
  • Gokulakrishnan Murugesan,
  • Rajath N. Rao,
  • Raja Selvaraj,
  • Ramesh Vinayagam,
  • S. M. Vidya

摘要

The present work reports the secretion of biosurfactant by probiotic Lactobacillus plantarum SVP2 (SVP2-BS) in a previously formulated fermented EPS rich milk whey beverage. The cell-free BS-SVP2 was extracted from the fermented milk whey beverage by acid precipitation giving yield of 9.62 g/L. The surface of SVP2-BS was fibrous network-like with presence of elemental carbon, oxygen and nitrogen in high amounts, as analysed by SEM-EDAX. SVP2-BS was confirmed as a lipoprotein by quantitative analysis and FT-IR characterization which revealed the presence of N-H, C = C, C-H2, C-O-C, C-O and C-H functional groups. The biofilm development of P. aeruginosa and E. coli was hindered by 96 ± 1.92% and 57 ± 1.14% on Ti-6Al-4 V alloy plates treated with 1.0 g/L SVP2-BS in comparison with untreated control plates. Additionally, SVP2-BS exhibited anti-cancer effects against HCT 116 colorectal cell lines (IC50:20 µg/mL), whereas exposure at 1000 µg/mL on IEC 6 mouse non-tumor epithelial cell lines, a viability of 60.2% was retained. The novelty of this study is that it reports the production of a bio-active biosurfactant with potent anti-biofilm and anti-cancer activity in a probiotic fermented bioactive EPS rich milk why based beverage, offering the consumer triple benefits of probiotic L. plantarum SVP2, its bioactive EPS and biosurfactant.