Scholarlink

<p><i>Lacticaseibacillus paracasei</i> is a typical food spoilage bacterium especially in beer. Due to the negative impact of <i>L. paracasei</i> on food preservation, a rapid detection method for <i>L. paracasei</i> based on polymerase spiral reaction (PSR) was established and its application was verified using different types of food samples in this study. Firstly, target digging was performed using bioinformatics techniques. A target gene <i>pheS</i> was selected and species-specific primers were designed. Secondly, the PSR assay was established with chromogenic reaction, which avoid false positive result due to open lid. The specificity of target and primers was tested, and the limit of detection (Kolodkin-Gal in Science 328:627–629, 2010) (5.2 × 10<sup>4</sup>&#xa0;fg/μL) was examined. Thirdly, the application of PSR assay in solid and liquid food samples were verified by artificial contamination model. The PSR assay was more efficiency in liquid food (LOD: 10<sup>3</sup>&#xa0;CFU/mL) than in solid food (LOD: 10<sup>4</sup>&#xa0;CFU/mL). Thus, <i>L. paracasei</i> screening in 3 types of beer (LOD: 10<sup>3</sup>&#xa0;CFU/mL) was further performed to verify to efficiency of PSR assay in screening of beer spoilage bacteria. In conclusion, <i>pheS</i> targeting PSR assay is useful as a rapid detection method for <i>L. paracasei</i> in solid and liquid food samples especially in beer.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Establishment and verification of a rapid detection method for Lacticaseibacillus paracasei based on polymerase spiral reaction (PSR): further application on screening of beer spoilage bacteria

  • 期刊论文

  • 发表日期：

    2025年5月23日

查看全文
  • Zhenbo Xu,
  • Rong Chen,
  • Jie Wu,
  • Feifeng Zhong,
  • Haoyue Xue,
  • Zhufang Xiang,
  • He Yang,
  • Lei Yuan,
  • Junyan Liu,
  • Junhong Yu

摘要

Lacticaseibacillus paracasei is a typical food spoilage bacterium especially in beer. Due to the negative impact of L. paracasei on food preservation, a rapid detection method for L. paracasei based on polymerase spiral reaction (PSR) was established and its application was verified using different types of food samples in this study. Firstly, target digging was performed using bioinformatics techniques. A target gene pheS was selected and species-specific primers were designed. Secondly, the PSR assay was established with chromogenic reaction, which avoid false positive result due to open lid. The specificity of target and primers was tested, and the limit of detection (Kolodkin-Gal in Science 328:627–629, 2010) (5.2 × 104 fg/μL) was examined. Thirdly, the application of PSR assay in solid and liquid food samples were verified by artificial contamination model. The PSR assay was more efficiency in liquid food (LOD: 103 CFU/mL) than in solid food (LOD: 104 CFU/mL). Thus, L. paracasei screening in 3 types of beer (LOD: 103 CFU/mL) was further performed to verify to efficiency of PSR assay in screening of beer spoilage bacteria. In conclusion, pheS targeting PSR assay is useful as a rapid detection method for L. paracasei in solid and liquid food samples especially in beer.