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<p>This study was conducted with the aim of producing micro-tissue from muscle (SBM) and fin (SBF) cell lines of Asian seabass, <i>Lates calcarifer</i>. These two cell lines were developed by explant culture method and sub-cultured for 65 passages for muscle cell line and 73 passages for fin cell line. They were successfully cryopreserved and 85–90% of cells were recovered after storage. Immunotyping results revealed that the cells of muscle and fin showed the presence of myosin, and that these cells might have originated from myoblasts. Amplification of mitochondrial large subunit ribosomal RNA gene specific to <i>L. calcarifer</i> revealed that these cell lines were derived from <i>L. calcarifer</i>. Both cell lines are capable of expressing foreign gene with 12–17% transfection efficiency. Sterilized polymer jelly beads and seaweed biofilm were used as scaffolds to culture muscle and fin cell lines. Cytotoxicity of direct and indirect media extract of both scaffolds was evaluated on SBM and SBF cell lines. Both scaffolds were found to be nontoxic and biocompatible for the growth of muscle and fin cells. Micro-tissue formation was observed on both scaffolds after 30 days of culture of these cell lines. Histological examination of tissue formed on polymer jelly bead showed 8–12 layers of cells on the surface of the beads, whereas more than 50 layers of muscle or fin cells were observed on seaweed biofilm. This low-cost seaweed biofilm is an environmentally biocompatible scaffold that could be used for the production of cell-based fish meat and therapeutic fish proteins.</p>

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Production of cell culture-based micro-tissue from muscle and fin cell lines of Lates calcarifer using biocompatible scaffolds

  • 期刊论文

  • 发表日期：

    2025年2月5日

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  • Sivaraj Mithra,
  • Seepoo Abdul Majeed,
  • Venkatesan Rajkumar,
  • Allahbagash Badhusha,
  • Gani Taju,
  • Azeez Sait Sahul Hameed

摘要

This study was conducted with the aim of producing micro-tissue from muscle (SBM) and fin (SBF) cell lines of Asian seabass, Lates calcarifer. These two cell lines were developed by explant culture method and sub-cultured for 65 passages for muscle cell line and 73 passages for fin cell line. They were successfully cryopreserved and 85–90% of cells were recovered after storage. Immunotyping results revealed that the cells of muscle and fin showed the presence of myosin, and that these cells might have originated from myoblasts. Amplification of mitochondrial large subunit ribosomal RNA gene specific to L. calcarifer revealed that these cell lines were derived from L. calcarifer. Both cell lines are capable of expressing foreign gene with 12–17% transfection efficiency. Sterilized polymer jelly beads and seaweed biofilm were used as scaffolds to culture muscle and fin cell lines. Cytotoxicity of direct and indirect media extract of both scaffolds was evaluated on SBM and SBF cell lines. Both scaffolds were found to be nontoxic and biocompatible for the growth of muscle and fin cells. Micro-tissue formation was observed on both scaffolds after 30 days of culture of these cell lines. Histological examination of tissue formed on polymer jelly bead showed 8–12 layers of cells on the surface of the beads, whereas more than 50 layers of muscle or fin cells were observed on seaweed biofilm. This low-cost seaweed biofilm is an environmentally biocompatible scaffold that could be used for the production of cell-based fish meat and therapeutic fish proteins.