<p>This study aims to evaluate the effects of different dedicated lane (DL) strategies on the performance and safety of highways in mixed traffic conditions where manually driven vehicles (MDVs) coexist with connected autonomous vehicles (CAVs). Using simulations, various DL strategies are examined in conjunction with market penetration rates (MPRs) and flow/capacity (v/c) ratios. Our findings indicate that an increase in the number of DLs reduces conflicts between CAV-MDV and CAV-CAV but increases conflicts among MDVs. Furthermore, it is noted that high v/c ratios worsen conflicts and diminish the positive effects of DLs. The effectiveness of the DL strategy becomes more prominent, particularly at high MPRs in highway segments outside of entrance and exit regions, while its impact is less in those areas. This study demonstrates that the DL strategy enhances safety by reducing collision risks between CAVs and MDVs, but its effectiveness is linked to MPR. Given its regional performance and safety analysis compared to existing studies on DL strategies, this study is anticipated to provide a significant contribution academically and practically.</p>