<p>Intersections of roads and wildlife movement pathways can lead to wildlife road mortality, resulting in population-level impacts. Wetland-road crossings are vulnerable locations for freshwater turtles that conduct inter- and intra-wetland movements during their active period to mate, forage, and nest. Identifying turtle road mortality hot spots may enable managers to implement mitigation efforts, such as locating and designing eco-passages. We assessed a rubric that predicts turtle road mortality risk by observing eighteen wetland-road crossing sites classified and ranked by the rubric. For eleven weeks during the turtles’ active period (May-July), we surveyed sites for road mortality and used cameras to observe movements across the road of four species: Blanding’s turtles (<i>Emydoidea blandingii</i>), spotted turtles (<i>Clemmys gutta</i>), snapping turtles (<i>Chelydra serpentina</i>), and eastern painted turtles (<i>Chrysemys picta</i>). Using negative binomial regressions, we demonstrate that the rubric identifies sites with higher rates of turtle road mortality (<i>p</i> &lt; 0.01) and that road accessibility is a significant factor in turtle road mortality rates (<i>p</i> = 0.02). Fewer turtles were observed on the road above wetland-road crossing structures (i.e., culverts) with larger openness ratios (<i>p</i> = 0.06). These findings may inform the design of road mortality mitigation structures, such as eco-passages, fencing, guide walls, and driver-awareness mechanisms. We also provide a conceptual engineering design to demonstrate how to reduce road accessibility and expand openness ratios to mitigate turtle mortality in a case study of one of our highest-risk sites.</p>