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<p>Marine seaweeds are rich in bioactive proteins with immense potential in foodomics, contributing to global food security and nutraceutical development. This review examines proteomic methodologies for studying seaweed proteins, focusing on their significance in functional food innovation. Proteomics, the broad-scale study of proteins, includes low-throughput techniques like 2D gel electrophoresis and western blotting, which provide detailed insights but are limited in scope. High-throughput methods, such as mass spectrometry and protein microarrays, enable large-scale protein profiling essential for exploring seaweed proteomes. Advanced approaches enhance accuracy and sensitivity, with bottom-up proteomics analyzing peptide fragments and middle-down and top-down methods preserving structural integrity for deeper insights. Hybrid instruments, integrating ion mobility spectrometry with mass spectrometry, further improve resolution and throughput. These advancements enable comprehensive characterization of seaweed-derived proteins, unlocking their roles in foodomics and driving sustainable, health-focused food innovations.</p>

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Low-high throughput and advanced proteomic analysis of marine seaweeds in foodomics

  • 期刊论文

  • 发表日期：

    2026年2月1日

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  • Sobia Naseem,
  • Muhammad Rizwan

摘要

Marine seaweeds are rich in bioactive proteins with immense potential in foodomics, contributing to global food security and nutraceutical development. This review examines proteomic methodologies for studying seaweed proteins, focusing on their significance in functional food innovation. Proteomics, the broad-scale study of proteins, includes low-throughput techniques like 2D gel electrophoresis and western blotting, which provide detailed insights but are limited in scope. High-throughput methods, such as mass spectrometry and protein microarrays, enable large-scale protein profiling essential for exploring seaweed proteomes. Advanced approaches enhance accuracy and sensitivity, with bottom-up proteomics analyzing peptide fragments and middle-down and top-down methods preserving structural integrity for deeper insights. Hybrid instruments, integrating ion mobility spectrometry with mass spectrometry, further improve resolution and throughput. These advancements enable comprehensive characterization of seaweed-derived proteins, unlocking their roles in foodomics and driving sustainable, health-focused food innovations.