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<p><i>Cryptocaryon irritans</i> is the parasite responsible for “white spot disease” in the yellowfin seabream <i>Acanthopagrus latus</i>, which has caused significant losses to the aquaculture industry. This experiment investigated the changes in the morphology, transcriptome, and proteome of gill tissue in yellowfin seabream infected with <i>C. irritans</i>, aiming to provide foundational data for further understanding the pathogenic mechanisms and control measures against this parasite. The main findings were as follows, after <i>C. irritans</i> infection, the structure of gill tissue in yellowfin seabream was damaged, with microvessels ruptured, some cells proliferating, and large amounts of mucus infiltrating. Transcriptome analysis revealed a total of 4 299 differentially expressed genes, with 2 367 up-regulated and 1 932 down-regulated. Further bioinformatics analysis of all differentially expressed genes identified nine immune-related genes, <i>cox-2, mcama, tbx21, dcn, tnfb, cd74a, illb, ppib</i>, and <i>cd4-1</i> in <i>A. latus</i>. The reliability of the transcriptome data was validated by real time qPCR, which showed the same trend as the RNA-seq results. Proteome analysis found 365 differential proteins, with 180 proteins up-regulated and 185 proteins down-regulated. Bioinformatics analysis identified three immune-related proteins, Myll, Gapdh, and Actn3b. These findings indicated that <i>C. irritans</i> disrupted the structure of gill tissue, impeded gas exchange and led to asphyxiation and death in affected fish. Transcriptome and proteome analyses showed that the expression of immune-related genes and proteins in yellowfin seabream, involved not only inflammatory responses and the activation and migration of immune cells but also potentially participated in tissue repair and defense regulation. These findings highlighted the complex immune regulatory network in response to <i>C. irritans</i> infection, offering references for prevention and control of white spot disease in yellowfin seabream.</p>

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Morphology, transcriptome, and proteome analysis of gill tissue in Acanthopagrus latus infected with Cryptocaryon irritans

  • 期刊论文

  • 发表日期：

    2025年9月1日

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  • Luxin Bai,
  • Man Jiang,
  • Dongling Zhang,
  • Jing Zhang,
  • Liangmin Huang,
  • Xiande Liu

摘要

Cryptocaryon irritans is the parasite responsible for “white spot disease” in the yellowfin seabream Acanthopagrus latus, which has caused significant losses to the aquaculture industry. This experiment investigated the changes in the morphology, transcriptome, and proteome of gill tissue in yellowfin seabream infected with C. irritans, aiming to provide foundational data for further understanding the pathogenic mechanisms and control measures against this parasite. The main findings were as follows, after C. irritans infection, the structure of gill tissue in yellowfin seabream was damaged, with microvessels ruptured, some cells proliferating, and large amounts of mucus infiltrating. Transcriptome analysis revealed a total of 4 299 differentially expressed genes, with 2 367 up-regulated and 1 932 down-regulated. Further bioinformatics analysis of all differentially expressed genes identified nine immune-related genes, cox-2, mcama, tbx21, dcn, tnfb, cd74a, illb, ppib, and cd4-1 in A. latus. The reliability of the transcriptome data was validated by real time qPCR, which showed the same trend as the RNA-seq results. Proteome analysis found 365 differential proteins, with 180 proteins up-regulated and 185 proteins down-regulated. Bioinformatics analysis identified three immune-related proteins, Myll, Gapdh, and Actn3b. These findings indicated that C. irritans disrupted the structure of gill tissue, impeded gas exchange and led to asphyxiation and death in affected fish. Transcriptome and proteome analyses showed that the expression of immune-related genes and proteins in yellowfin seabream, involved not only inflammatory responses and the activation and migration of immune cells but also potentially participated in tissue repair and defense regulation. These findings highlighted the complex immune regulatory network in response to C. irritans infection, offering references for prevention and control of white spot disease in yellowfin seabream.