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<p>Low iron content is a peculiar feature of marine ecosystems, where microbes have to produce iron-chelating molecules such as siderophores to survive. Very little is known about siderophore-producing bacteria in the oceans. In this study, we screened 1546 strains from marine seawater and sediments, which were deposited in the Marine Culture Collection of China (MCCC), and further analyzed the diversity of positive strains and their potential genes related to iron acquisition. Of the 1546 isolates, 856 strains (55.37%) showed positive siderophore-producing activity on the Chrome Azurol Sulfonate (CAS) plates. Among these, isolates from seawater environments had a higher positive proportion (535). Some genera showed a higher proportion (&gt;70%) of positive siderophore producers, such as <i>Alteromonas</i> (89/112), <i>Marinobacter</i> (78/109), <i>Vibrio</i> (21/27), <i>Shewanella</i> (7/8) in the Gammaproteobacteria, <i>Sulfitobacter</i> (17/21), <i>Martelella</i> (5/6) in the Alphaproteobacteria, and <i>Joostella</i> (6/7) in the phylum Bacteroidetes. Siderophore biosynthesis genes, including those for vanchrobactin, vibrioferrin, petrobactin, and aerobactin, as well as transport and iron storage proteins, were also identified in the positive bacterial genomes. The study revealed that a variety of bacterial strains demonstrate the production of siderophores, which could significantly contribute to the iron cycle within marine ecosystems, encompassing both seawater and marine sediments.</p>

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Screening of siderophore-producing bacteria isolated from marine environments

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • Xiang Zeng,
  • Guangyu Li,
  • Yaping Du,
  • Tong Yu,
  • Qiliang Lai,
  • Liping Wang,
  • Zongze Shao

摘要

Low iron content is a peculiar feature of marine ecosystems, where microbes have to produce iron-chelating molecules such as siderophores to survive. Very little is known about siderophore-producing bacteria in the oceans. In this study, we screened 1546 strains from marine seawater and sediments, which were deposited in the Marine Culture Collection of China (MCCC), and further analyzed the diversity of positive strains and their potential genes related to iron acquisition. Of the 1546 isolates, 856 strains (55.37%) showed positive siderophore-producing activity on the Chrome Azurol Sulfonate (CAS) plates. Among these, isolates from seawater environments had a higher positive proportion (535). Some genera showed a higher proportion (>70%) of positive siderophore producers, such as Alteromonas (89/112), Marinobacter (78/109), Vibrio (21/27), Shewanella (7/8) in the Gammaproteobacteria, Sulfitobacter (17/21), Martelella (5/6) in the Alphaproteobacteria, and Joostella (6/7) in the phylum Bacteroidetes. Siderophore biosynthesis genes, including those for vanchrobactin, vibrioferrin, petrobactin, and aerobactin, as well as transport and iron storage proteins, were also identified in the positive bacterial genomes. The study revealed that a variety of bacterial strains demonstrate the production of siderophores, which could significantly contribute to the iron cycle within marine ecosystems, encompassing both seawater and marine sediments.