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<p>This study evaluated the efficacy of adipose-derived mesenchymal stem cells (AD-MSCs) in reducing pathological alterations linked to acute kidney injury (AKI) caused by ischemia–reperfusion injury (IRI). In this study, 18 male rats were divided into three groups: Sham, IRI, and IRI + AD-MSC. IRI was induced by clamping the renal pedicles for 45 min, while the Sham group animals underwent the same surgical procedure without vascular clamping. In the IRI + AD-MSC groups, immediately after clamp removal, the animals received an intraperitoneal injection of 1 × 10⁶ AD-MSCs. To enable tracking, AD-MSCs were transfected with green fluorescent protein (GFP). The distribution of these labeled cells was examined at 24 and 48 h post-transplantation in three additional rats. Forty-eight hours after reperfusion, serum and kidney tissue samples were collected. The labeled AD-MSCs were more widely distributed in the injured kidney area of the IRI + AD-MSC group compared to the control group. In the group treated with AD-MSCs, there were notable reductions in serum blood urea nitrogen (BUN) and creatinine levels (<i>P</i> &lt; 0.001), along with improvements in renal tissue damage and oxidative stress (<i>P</i> &lt; 0.01). Additionally, the IRI + AD-MSC group showed significant decreases in toll-like receptor-4 (TLR4) protein levels (<i>P</i> &lt; 0.01) and pro-inflammatory factors (<i>P</i> &lt; 0.001), as well as reduced levels of endoplasmic reticulum (ER) stress proteins compared to the IRI group. This study suggested that AD-MSCs administration alleviates AKI, possibly by modulating TLR4 activity, decreasing inflammation, and reducing ER stress.</p>

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Adipose-derived mesenchymal stem cells improved acute renal failure induced by ischemia–reperfusion injury: Focusing on toll-like receptor 4 activity and endoplasmic reticulum stress response

  • 期刊论文

  • 发表日期：

    2026年7月11日

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  • Leila Hafazeh,
  • Mehri Kadkhodaee,
  • Behjat Seifi,
  • Fariba Akhondzadeh,
  • Fereshteh Azedi,
  • Hamid Noori,
  • Mahdi Hajiaqaei,
  • Mina Ranjbaran

摘要

This study evaluated the efficacy of adipose-derived mesenchymal stem cells (AD-MSCs) in reducing pathological alterations linked to acute kidney injury (AKI) caused by ischemia–reperfusion injury (IRI). In this study, 18 male rats were divided into three groups: Sham, IRI, and IRI + AD-MSC. IRI was induced by clamping the renal pedicles for 45 min, while the Sham group animals underwent the same surgical procedure without vascular clamping. In the IRI + AD-MSC groups, immediately after clamp removal, the animals received an intraperitoneal injection of 1 × 10⁶ AD-MSCs. To enable tracking, AD-MSCs were transfected with green fluorescent protein (GFP). The distribution of these labeled cells was examined at 24 and 48 h post-transplantation in three additional rats. Forty-eight hours after reperfusion, serum and kidney tissue samples were collected. The labeled AD-MSCs were more widely distributed in the injured kidney area of the IRI + AD-MSC group compared to the control group. In the group treated with AD-MSCs, there were notable reductions in serum blood urea nitrogen (BUN) and creatinine levels (P < 0.001), along with improvements in renal tissue damage and oxidative stress (P < 0.01). Additionally, the IRI + AD-MSC group showed significant decreases in toll-like receptor-4 (TLR4) protein levels (P < 0.01) and pro-inflammatory factors (P < 0.001), as well as reduced levels of endoplasmic reticulum (ER) stress proteins compared to the IRI group. This study suggested that AD-MSCs administration alleviates AKI, possibly by modulating TLR4 activity, decreasing inflammation, and reducing ER stress.