<p>Salinity causes a variety of problems for plants, especially salt-sensitive species like Soybean. Soybean (<i>Glycine max</i> L.) is an essential and vital oil seed crop around the world. Salinity lowers the soil’s water potential, which limits plants’ ability to absorb water and creates a water deficit. Osmotic stress and ionic toxicity caused by salinity lead to secondary oxidative stress in plants. Therefore, soil salinity harms crop physiology, growth, and yield. Hence, the current study was conducted to assess the developed tolerance potential of the germplasm obtained from previous studied parental seed of soybean cultivar JS-9560 and JS-335 treated with abscisic acid (ABA), salicylic acid (SA) and plant growth promoting bacteria (PGPB) under 50 and 100 ppm salinity. Result of the current study showed that the Salicylic acid (SA), abscisic acid (ABA), and plant growth-promoting bacteria (PGPB) work in concert to shield soybean (<i>Glycine max</i> L.) cultivars against salinity stress. These substances aid in transgenerational priming when applied to parent plants, enabling the offspring to display improved osmotic adjustment, increased stress tolerance, and more robust antioxidant defense mechanisms. Application of 100ppm salt in soil was highly detrimental than 50ppm salt concentration. However, study identified that the germplasm of the plants treated with exogenous protectants had enhanced growth, biomass accumulation, and yield performance. Antioxidative potential and solute accumulation of these germplasm plants were also higher while, Na⁺/ K⁺ ratio was lower than control plants. Growth and yield performance was maximum in the germplasm of the plants treated with PGPB followed by SA and ABA. Protein profiling of the plants showed high intensity emergence of two to three new protein bands at lower molecular weight in protectant treated germplasm could be the possible reason for enhanced salt tolerance in soybean. Presented study concluded that the application of exogenous protectant acquires salt tolerance in progeny. However, variation in response among both the cultivars suggested effectiveness of the treatment is cultivar dependent. Future potential for using salicylic acid (SA), abscisic acid (ABA), and plant growth-promoting bacteria (PGPB) on soybean (Glycine max L.) parent plants revolves around epigenetic priming. This makes it possible for progeny to become more naturally resistant to salinity stress.</p>