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<p>With the recent changes in the global climate, the repercussions of extreme climatic events have received increased attention worldwide. Drought is an important meteorological climatic disaster whose frequency, duration, and spatial extent are increasing and have a decisive impact on plant morphophysiological and biochemical responses. Several studies demonstrated that nitric oxide (NO) plays a significant role in various abiotic stresses in plants, but its genotype-specific role in lentil drought resilience remains insufficiently explored. Therefore, the present study investigated the comparative response of two contrasting lentil genotypes (drought-tolerant DPL-58 and drought-sensitive JL-3) under polyethylene glycol (PEG-6000)-induced drought stress (5% and 10%, simulating moderate and severe stress conditions). Results showed that drought stress significantly reduced growth (up to 40–80%), relative water content (27–58%), and chlorophyll content (46–75%), and increased oxidative damage (128–173%) in both genotypes. However, exogenous NO was supplied through sodium nitroprusside (SNP) at two concentrations (50 and 100&#xa0;μmol) to evaluate dose-dependent effects. SNP application, particularly at 100&#xa0;µmol, markedly improved growth (55–88%), fresh and dry biomass (39–168%), water status (46–89%), and photosynthetic pigments (65–169%), while reducing membrane damage (MDA accumulation up to 30–35%) and enhancing activities of antioxidant enzymes (SOD, CAT, and POD). JL-3 exhibited a more pronounced recovery under SNP treatment compared to DPL-58, indicating stronger NO-mediated responsiveness in the sensitive genotype. Multivariate analysis of the data revealed statistically significant differences among the treatments, whereas the correlation coefficient matrix and principal component analysis accounted for the variance (96.99% in DPL-58 and 95.51% in JL-3) in the data due to SNP application. Conclusively, this study provides novel insights into the genotype-dependent and dose-specific role of NO in lentil, highlighting its potential as a sustainable strategy to improve drought resilience in lentil crops.</p> Graphical abstract <p>Conceptual outline exemplifying the main findings and possible mechanisms mediating improvement in the lentil plant following supplementation of nitric oxide under drought stress.</p>

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Exploring the restorative potential of nitric oxide on morpho-physiological and biochemical attributes of lentil genotypes under drought stress

  • 期刊论文

  • 发表日期：

    2026年7月1日

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  • Sakshi Saini,
  • Priyanka Sharma,
  • Jyoti Sharma,
  • Pooja Pooja,
  • Asha Sharma

摘要

With the recent changes in the global climate, the repercussions of extreme climatic events have received increased attention worldwide. Drought is an important meteorological climatic disaster whose frequency, duration, and spatial extent are increasing and have a decisive impact on plant morphophysiological and biochemical responses. Several studies demonstrated that nitric oxide (NO) plays a significant role in various abiotic stresses in plants, but its genotype-specific role in lentil drought resilience remains insufficiently explored. Therefore, the present study investigated the comparative response of two contrasting lentil genotypes (drought-tolerant DPL-58 and drought-sensitive JL-3) under polyethylene glycol (PEG-6000)-induced drought stress (5% and 10%, simulating moderate and severe stress conditions). Results showed that drought stress significantly reduced growth (up to 40–80%), relative water content (27–58%), and chlorophyll content (46–75%), and increased oxidative damage (128–173%) in both genotypes. However, exogenous NO was supplied through sodium nitroprusside (SNP) at two concentrations (50 and 100 μmol) to evaluate dose-dependent effects. SNP application, particularly at 100 µmol, markedly improved growth (55–88%), fresh and dry biomass (39–168%), water status (46–89%), and photosynthetic pigments (65–169%), while reducing membrane damage (MDA accumulation up to 30–35%) and enhancing activities of antioxidant enzymes (SOD, CAT, and POD). JL-3 exhibited a more pronounced recovery under SNP treatment compared to DPL-58, indicating stronger NO-mediated responsiveness in the sensitive genotype. Multivariate analysis of the data revealed statistically significant differences among the treatments, whereas the correlation coefficient matrix and principal component analysis accounted for the variance (96.99% in DPL-58 and 95.51% in JL-3) in the data due to SNP application. Conclusively, this study provides novel insights into the genotype-dependent and dose-specific role of NO in lentil, highlighting its potential as a sustainable strategy to improve drought resilience in lentil crops.

Graphical abstract

Conceptual outline exemplifying the main findings and possible mechanisms mediating improvement in the lentil plant following supplementation of nitric oxide under drought stress.