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<p>Culm strength is a key determinant of lodging resistance in rice, directly impacting yield stability and resilience. To identify genomic regions associated with strong culm, we characterized the strong culm mutant TI-17, derived from the Indian mega rice variety <i>Samba Mahsuri (BPT-5204)</i>. Comparative anatomical analyses revealed that TI-17 exhibited increased culm wall thickness, sclerenchyma thickness, and xylem vessel sclerenchyma thickness, along with a reduced xylem vessel diameter relative to BPT-5204. Using the MutMap approach, we identified 12 candidate genes distributed across three genomic regions on chromosomes 3 (32.71–33.70&#xa0;Mb), 4 (18.2–20.0&#xa0;Mb), and 8 (24.0–27.0&#xa0;Mb). SNPs within the candidate regions were converted into KASP assays, including KASP8_9 (chr8:26248706; A→G; CAG [Glutamine] to CGG [Arginine]) in <i>Os08g0527300</i>, encoding peptidase C19, ubiquitin carboxyl-terminal hydrolase 2 domain containing protein. This SNP of KASP8_9 demonstrated perfect co-segregation with culm strength, effectively differentiating weak and strong culm genotypes. The findings highlight <i>Os08g0527300</i> as a promising target for the genetic improvement of culm strength, contributing to the development of high-yield, climate-resilient rice varieties.</p>

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Next generation sequencing-based MutMap identifies genomic regions associated with strong culm in rice

  • 期刊论文

  • 发表日期：

    2026年6月18日

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  • Pritam Kanti Guha,
  • Anil A. Hake,
  • Kalyani M. Barbadikar,
  • Potupureddi Gopi,
  • Nakul D. Magar,
  • Vishalakshi Balija,
  • C. G. Gokulan,
  • Madhavilatha Kommana,
  • Md Jamaloddin,
  • Anjana Sharma,
  • Raju Madanala,
  • A. Chandra Sekhar,
  • D. Vijaya Raghava Prasad,
  • D. Vijaya Lakshmi,
  • Hitendra Kumar Patel,
  • Ramesh Venkata Sonti,
  • Sundaram Raman Meenakshi,
  • Sheshu Madhav Maganti

摘要

Culm strength is a key determinant of lodging resistance in rice, directly impacting yield stability and resilience. To identify genomic regions associated with strong culm, we characterized the strong culm mutant TI-17, derived from the Indian mega rice variety Samba Mahsuri (BPT-5204). Comparative anatomical analyses revealed that TI-17 exhibited increased culm wall thickness, sclerenchyma thickness, and xylem vessel sclerenchyma thickness, along with a reduced xylem vessel diameter relative to BPT-5204. Using the MutMap approach, we identified 12 candidate genes distributed across three genomic regions on chromosomes 3 (32.71–33.70 Mb), 4 (18.2–20.0 Mb), and 8 (24.0–27.0 Mb). SNPs within the candidate regions were converted into KASP assays, including KASP8_9 (chr8:26248706; A→G; CAG [Glutamine] to CGG [Arginine]) in Os08g0527300, encoding peptidase C19, ubiquitin carboxyl-terminal hydrolase 2 domain containing protein. This SNP of KASP8_9 demonstrated perfect co-segregation with culm strength, effectively differentiating weak and strong culm genotypes. The findings highlight Os08g0527300 as a promising target for the genetic improvement of culm strength, contributing to the development of high-yield, climate-resilient rice varieties.