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<p>The study was aimed to optimize a tissue culture regeneration protocol for banana cv. Grand Naine and comparing the performance of tissue culture (TC) and conventionally grown sucker plants under field conditions. Three different plant growth regulators (PGRs) viz., Thidiazuron (TDZ), benzyl amino purine (BAP), and kinetin with 10 combinations at varying concentrations were put in the MS media to evaluate days to shoot and root emergence, shoot and root length and number of shoots and roots. Data were taken 2 months after culturing the explants in the media. TDZ at 0.5&#xa0;mg L<sup>− 1</sup> was the optimum concentration showing the most effective root and shoot induction. Following acclimatization, TC plants and sucker-derived plants were grown under two fertilizer treatments (N: P:K at 300:50:300 and 400:60:400&#xa0;g/plant) for comparative assessment based on growth, yield, fruit quality, and economic returns. TC plants showed earlier shooting and bunch development, more number of functional leaves, better yield attributing characters and better fruit quality with higher TSS content and less titratable acidity. However, sucker-derived plantlets had marginally better fruit shelf life. The pulp to peel ratio did not vary significantly among the TC propagated and sucker propagated plants. TC plants showed a significantly higher benefit-cost ratio, reflecting better economic feasibility. The study confirmed that TC propagation maintaining a standardized protocol provided uniform, disease-free planting material and improved crop performance and profitability. This integrated approach acts as a sustainable solution for enhancing banana cultivation and profitability.</p>

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Standardizing in-vitro propagation and evaluating field performance against conventional sucker propagation in banana (Musa spp. cv. Grand Naine)

  • 期刊论文

  • 发表日期：

    2026年1月20日

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  • Rajdeep Mohanta,
  • Souvik Chakraborty,
  • Soham Hazra,
  • Payal Maiti,
  • Sourav Roy,
  • Subhadwip Ghorai,
  • Fatik Kumar Bauri

摘要

The study was aimed to optimize a tissue culture regeneration protocol for banana cv. Grand Naine and comparing the performance of tissue culture (TC) and conventionally grown sucker plants under field conditions. Three different plant growth regulators (PGRs) viz., Thidiazuron (TDZ), benzyl amino purine (BAP), and kinetin with 10 combinations at varying concentrations were put in the MS media to evaluate days to shoot and root emergence, shoot and root length and number of shoots and roots. Data were taken 2 months after culturing the explants in the media. TDZ at 0.5 mg L− 1 was the optimum concentration showing the most effective root and shoot induction. Following acclimatization, TC plants and sucker-derived plants were grown under two fertilizer treatments (N: P:K at 300:50:300 and 400:60:400 g/plant) for comparative assessment based on growth, yield, fruit quality, and economic returns. TC plants showed earlier shooting and bunch development, more number of functional leaves, better yield attributing characters and better fruit quality with higher TSS content and less titratable acidity. However, sucker-derived plantlets had marginally better fruit shelf life. The pulp to peel ratio did not vary significantly among the TC propagated and sucker propagated plants. TC plants showed a significantly higher benefit-cost ratio, reflecting better economic feasibility. The study confirmed that TC propagation maintaining a standardized protocol provided uniform, disease-free planting material and improved crop performance and profitability. This integrated approach acts as a sustainable solution for enhancing banana cultivation and profitability.