<p>The present study reports a callus-mediated indirect regeneration protocol of potato cultivar Badami alu, an early-maturing, reniform, reddish-purple tuber with superior red starch quality, for the first time. Internodal explants (surface disinfected using Tween-20^®, sodium hypochlorite, cetrimide, and ethyl alcohol) were inoculated on Murashige and Skoog (MS) medium supplemented with varying concentrations and combinations of 6-benzyladenine (BA), α-naphthaleneacetic acid (NAA), kinetin (Kin), and 2,4-dichlorophenoxyacetic acid (2,4-D). Out of these plant growth regulators, 2.0 mg L⁻¹ BA + 1.0 mg L⁻¹ NAA resulted in 100% callus induction, maximum fresh weight (28.6&#xa0;mg) and dry weight (1.7&#xa0;mg), and area (73.6 sq. mm) of callus. The earliest (7.5 days) callus induction was observed in 1.5 mg L⁻¹ BA + 1.0 mg L⁻¹ NAA-supplemented medium. During indirect regeneration from calli, the combination of 2.0 mg L⁻¹ BA and 0.1 mg L⁻¹ NAA resulted in a comparatively greater number and length of shoots, roots, and number of leaves; whereas the combination of 1.0 mg L⁻¹ Kin and 0.1 mg L⁻¹ NAA induced the earliest shoot and root development. The histological analysis provided well-defined structures of friable, compact, and organogenic tissue of the calli. Flow cytometry and PCR-based molecular marker analyses confirmed the stable ploidy level and genetic fidelity of regenerated plantlets with their mother plant. The developed protocol should be of immense potential for mass production of plantlets and genetic transformation studies on the cultivar Badami alu.</p>