Scholarlink

<p>Barley (<i>Hordeum vulgare</i> L. ssp. <i>vulgare</i>) is the world’s fourth most important cereal, primarily used for animal feed and malting. Various barley varieties have been developed to meet these distinct uses and adapt to diverse environments. However, increasing climate change pressures underscore the need to enhance crop resilience. Induced mutagenesis using ethyl methanesulfonate (EMS) is a well-established strategy for increasing genetic variability and selecting for key traits. This study aimed to determine the optimal EMS treatment in malting and forage barley for the selection of drought-resistant mutants. Seeds underwent EMS treatments at varying concentrations (0.1–1.0% v/v) and exposure times (1–16&#xa0;h), assessing germination, survival, shoot and root length, biomass, and seedling vigor. The optimal EMS treatment was 0.5% for 2.7&#xa0;h in the forage variety and 2.6&#xa0;h in the malting variety. A set of mutants was subsequently subjected to drought stress; ten forage mutants survived, whereas none from the malting group did. Further selection based on leaf water loss identified three promising forage mutants (For-7, For-8, and For-10), highlighting their potential for breeding drought-tolerant barley. These findings advance our understanding of mutagenesis in barley and support future efforts to enhance stress tolerance in this vital crop.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Enhancing drought tolerance in malting and forage barley through mutagenesis

  • 期刊论文

  • 发表日期：

    2025年7月25日

查看全文
  • Dianey Celeste Cruz-Muñoz,
  • Myriam Guadalupe Rodríguez-Gandarilla,
  • Miguel Angel Avila-Perches,
  • Rafael Urrea-López,
  • Julio Armando Massange-Sánchez

摘要

Barley (Hordeum vulgare L. ssp. vulgare) is the world’s fourth most important cereal, primarily used for animal feed and malting. Various barley varieties have been developed to meet these distinct uses and adapt to diverse environments. However, increasing climate change pressures underscore the need to enhance crop resilience. Induced mutagenesis using ethyl methanesulfonate (EMS) is a well-established strategy for increasing genetic variability and selecting for key traits. This study aimed to determine the optimal EMS treatment in malting and forage barley for the selection of drought-resistant mutants. Seeds underwent EMS treatments at varying concentrations (0.1–1.0% v/v) and exposure times (1–16 h), assessing germination, survival, shoot and root length, biomass, and seedling vigor. The optimal EMS treatment was 0.5% for 2.7 h in the forage variety and 2.6 h in the malting variety. A set of mutants was subsequently subjected to drought stress; ten forage mutants survived, whereas none from the malting group did. Further selection based on leaf water loss identified three promising forage mutants (For-7, For-8, and For-10), highlighting their potential for breeding drought-tolerant barley. These findings advance our understanding of mutagenesis in barley and support future efforts to enhance stress tolerance in this vital crop.