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<p>Leporids play an important ecological role by contributing to seed dispersal and serving as prey for a wide range of predators. However, several leporid species are threatened by habitat degradation, highlighting the need for genetic resources that can support studies of wild and managed populations. The SRY gene, located on the Y chromosome in most male mammals, is a useful marker for paternal lineage studies and may provide information relevant to conservation genetics. Here, we generated partial Sex-determining region Y (SRY) sequences from 14 male leporids: 12 zoo-managed <i>Romerolagus diazi</i>, one wild <i>R. diazi</i> carcass, and one wild <i>Sylvilagus floridanus</i> carcass. Three unique consensus sequences were obtained and deposited in GenBank: PV612454 and PV612455 for <i>R. diazi</i>, and PV612456 for <i>S. floridanus</i>. The analyzed fragments comprised 703 base pairs (bp) in <i>R. diazi</i> and 741&#xa0;bp in <i>S. floridanus</i>, including approximately 173&#xa0;bp of the 5′ regulatory region and a partial transcriptional unit covering the first 176 codons, including the high-mobility group box domain; the complete SRY coding region was not recovered. Comparisons with available leporid SRY sequences showed that the regulatory region had lower guanine-cytosine content than the transcriptional unit, whereas <i>R. diazi</i> and <i>S. floridanus</i> showed relatively high guanine-cytosine values in the regulatory and 3′ SRY regions. Polymorphic sites were detected in the regulatory and coding regions, and amino acid variation was concentrated outside the conserved high-mobility group box domain. A distance-based, single-locus comparison provided a preliminary paternal-lineage framework among the leporid sequences analyzed.</p>

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Partial SRY gene sequences of the endangered volcano rabbit (Romerolagus diazi) and the eastern cottontail rabbit (Sylvilagus floridanus) as Y-linked molecular resources for leporid conservation genetics

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月18日

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  • Raúl Ulloa-Arvizu,
  • José Antonio Sandoval Zarate,
  • Rogelio Campos Morales,
  • Fernando Chávez-Maya

摘要

Leporids play an important ecological role by contributing to seed dispersal and serving as prey for a wide range of predators. However, several leporid species are threatened by habitat degradation, highlighting the need for genetic resources that can support studies of wild and managed populations. The SRY gene, located on the Y chromosome in most male mammals, is a useful marker for paternal lineage studies and may provide information relevant to conservation genetics. Here, we generated partial Sex-determining region Y (SRY) sequences from 14 male leporids: 12 zoo-managed Romerolagus diazi, one wild R. diazi carcass, and one wild Sylvilagus floridanus carcass. Three unique consensus sequences were obtained and deposited in GenBank: PV612454 and PV612455 for R. diazi, and PV612456 for S. floridanus. The analyzed fragments comprised 703 base pairs (bp) in R. diazi and 741 bp in S. floridanus, including approximately 173 bp of the 5′ regulatory region and a partial transcriptional unit covering the first 176 codons, including the high-mobility group box domain; the complete SRY coding region was not recovered. Comparisons with available leporid SRY sequences showed that the regulatory region had lower guanine-cytosine content than the transcriptional unit, whereas R. diazi and S. floridanus showed relatively high guanine-cytosine values in the regulatory and 3′ SRY regions. Polymorphic sites were detected in the regulatory and coding regions, and amino acid variation was concentrated outside the conserved high-mobility group box domain. A distance-based, single-locus comparison provided a preliminary paternal-lineage framework among the leporid sequences analyzed.