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<p>We propose an approach of 6 STR-loci to one-stage species identification of four species of the <i>Canidae</i> family, the red fox (<i>Vulpes vulpes</i>), the arctic fox (<i>Vulpes lagopus</i>), the raccoon dog (<i>Nyctereutes procyonoides</i>), and the wolf (including the grey wolf (<i>Canis lupus lupus</i>) and the domestic dog (<i>Canis lupus familiaris</i>), based on differences in manifestation of microsatellite loci during cross-species amplification. The test system was optimized for interspecies differentiation within the <i>Canidae</i> family rather than identification of individual animals. Additionally, we present an approach to DNA identification of the subspecies of the <i>Canis lupus</i>, the grey wolf (<i>Canis lupus lupus</i>) and the domestic dog (<i>Canis lupus familiaris</i>), based on variations in the copy number of the amylase gene (<i>Amy2b</i>). This comprehensive study of canids makes it possible to reliably differentiate forensic samples by species and subspecies before genetic identification of individuals.</p>

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A new approach to the DNA-identification of species and subspecies of Canidae in forensic studies

  • 期刊论文

  • 发表日期：

    2026年2月21日

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  • Aliaksandra E. Hrebianchuk,
  • Nastassia S. Parfionava,
  • Iosif S. Tsybovsky

摘要

We propose an approach of 6 STR-loci to one-stage species identification of four species of the Canidae family, the red fox (Vulpes vulpes), the arctic fox (Vulpes lagopus), the raccoon dog (Nyctereutes procyonoides), and the wolf (including the grey wolf (Canis lupus lupus) and the domestic dog (Canis lupus familiaris), based on differences in manifestation of microsatellite loci during cross-species amplification. The test system was optimized for interspecies differentiation within the Canidae family rather than identification of individual animals. Additionally, we present an approach to DNA identification of the subspecies of the Canis lupus, the grey wolf (Canis lupus lupus) and the domestic dog (Canis lupus familiaris), based on variations in the copy number of the amylase gene (Amy2b). This comprehensive study of canids makes it possible to reliably differentiate forensic samples by species and subspecies before genetic identification of individuals.