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<p>To improve our understanding of the genetic diversity and population structure of <i>Metapenaeus intermedius</i> for germplasm resource conservation, we developed and characterized 67 polymorphic microsatellite markers for this species. The number of alleles per locus ranged from 3 to 17. The observed heterozygosity (<i>H</i><sub><i>O</i></sub>) varied from 0.2854 to 0.9875, whereas the expected heterozygosity (<i>H</i><sub><i>E</i></sub>) ranged from 0.2957 to 0.9327. Twenty-nine loci exhibited moderate polymorphism (0.25 &lt; <i>PIC</i> &lt; 0.5), while the remaining loci demonstrated high polymorphism (<i>PIC</i> &gt; 0.5). Furthermore, 10 loci deviated from the Hardy‒Weinberg equilibrium (<i>P</i><sub><i>HW</i></sub>). These markers will serve as valuable tools for population genetic analyses and the conservation of <i>M. intermedius</i>.</p>

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Characterization and development of 67 polymorphic microsatellite markers for Metapenaeus intermedius

  • 期刊论文

  • 发表日期：

    2026年2月13日

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  • Fahui Gong,
  • Jihe Zhao,
  • Zhihao Li,
  • Jingzhi Sui,
  • Qing Liu,
  • Yucui Shi,
  • Yuzhen Niu,
  • Shasha Zhao,
  • Guofu Huang,
  • Jimei Song,
  • Youxin Xu

摘要

To improve our understanding of the genetic diversity and population structure of Metapenaeus intermedius for germplasm resource conservation, we developed and characterized 67 polymorphic microsatellite markers for this species. The number of alleles per locus ranged from 3 to 17. The observed heterozygosity (HO) varied from 0.2854 to 0.9875, whereas the expected heterozygosity (HE) ranged from 0.2957 to 0.9327. Twenty-nine loci exhibited moderate polymorphism (0.25 < PIC < 0.5), while the remaining loci demonstrated high polymorphism (PIC > 0.5). Furthermore, 10 loci deviated from the Hardy‒Weinberg equilibrium (PHW). These markers will serve as valuable tools for population genetic analyses and the conservation of M. intermedius.