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<p>The impact of habitat loss on patterns of neutral and adaptive genetic variability in the rheophilic ichthyofauna has been little studied. Here, we used a ddRAD-seq approach on specimens of the genus Baryancistrus sampled from rheophilic habitats under strong anthropogenic influences, to identify geographically informative nuclear DNA single nucleotide polymorphisms (SNPs) loci. We explored different bioinformatic parameters and made datasets, with retrieved SNPs varying between 551 and 60,687. The filter with 40% of missing data indicated lower dispersion and more symmetry over the dataset. These SNPs datasets are now available and will be useful for large-scale genetic studies on this endemic and endangered Amazonic catfish.</p>

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Unveiling the genetic mysteries of Amazonian aquatic species: exploring the catfish genome through sequencing for SNP discovery in rheophilic species

  • 期刊论文

  • 发表日期：

    2025年9月13日

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  • Éder Cristian Malta de Lanes,
  • Iann Leonardo Pinheiro Monteiro,
  • Vanessa Paes da Cruz,
  • Silvia E. Pavan,
  • Alberto Akama

摘要

The impact of habitat loss on patterns of neutral and adaptive genetic variability in the rheophilic ichthyofauna has been little studied. Here, we used a ddRAD-seq approach on specimens of the genus Baryancistrus sampled from rheophilic habitats under strong anthropogenic influences, to identify geographically informative nuclear DNA single nucleotide polymorphisms (SNPs) loci. We explored different bioinformatic parameters and made datasets, with retrieved SNPs varying between 551 and 60,687. The filter with 40% of missing data indicated lower dispersion and more symmetry over the dataset. These SNPs datasets are now available and will be useful for large-scale genetic studies on this endemic and endangered Amazonic catfish.