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<p>Effective conservation of endangered species necessitates accurate population size and genetic diversity estimates. Individual identification is essential for understanding population dynamics, gene flow, and habitat use patterns. This study aimed to develop efficient tools for individual identification of otters by developing multiplex PCR-based microsatellite and sex-specific marker panels. A total of 11 stable microsatellite markers were selected from 41 previously reported markers and new primers were designed for sex determination. By analyzing 91 fecal samples collected from a specific section of the Banbyeoncheon stream in Youngyang-gun, South Korea, a total of 24 individuals were identified using developed markers. Genetic diversity analysis of the population showed a high level of genetic diversity, with observed heterozygosity of 0.7046 and expected heterozygosity of 0.7021. Multiplex PCR panels developed in this study can be utilized for otter population monitoring and conservation research, ultimately contributing to our understanding of habitat use characteristics of otter population.</p>

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Individual identification of Eurasian otters (Lutra lutra) in South Korea (Banbyeoncheon, Yeongyan) based on multiplex PCR analysis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月8日

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  • Da Som Park,
  • Yung kun Kim,
  • Hee-Bok Park,
  • KyungMin Park,
  • Keun-Sik Kim,
  • Kuem Hee Jang,
  • Seungki Lee,
  • Jeong Chan Moon

摘要

Effective conservation of endangered species necessitates accurate population size and genetic diversity estimates. Individual identification is essential for understanding population dynamics, gene flow, and habitat use patterns. This study aimed to develop efficient tools for individual identification of otters by developing multiplex PCR-based microsatellite and sex-specific marker panels. A total of 11 stable microsatellite markers were selected from 41 previously reported markers and new primers were designed for sex determination. By analyzing 91 fecal samples collected from a specific section of the Banbyeoncheon stream in Youngyang-gun, South Korea, a total of 24 individuals were identified using developed markers. Genetic diversity analysis of the population showed a high level of genetic diversity, with observed heterozygosity of 0.7046 and expected heterozygosity of 0.7021. Multiplex PCR panels developed in this study can be utilized for otter population monitoring and conservation research, ultimately contributing to our understanding of habitat use characteristics of otter population.