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<p>A disposable and non-enzymatic sensor had been constructed for determination of oxidized form of nicotinamide adenine dinucleotide (NAD<sup>+</sup>) in pig serum samples successfully. Single-walled carbon nanotubes (SWCNT) were immobilized on screen-printed carbon electrode (SPCE) by co-polymerized with monomer <i>o</i>-aminophenol (<i>o</i>-AP) (POAP) by cyclic voltammetry technique. Electrochemical impedance spectroscopy showed SWCNT/POAP/SPCE possesses a higher conductivity of 12.2 Ω⋅cm<sup>2</sup>, which is identified as an effective electrocatalyst for determining NAD<sup>+</sup>. A reduction peak at a potential of around <b>−</b>0.31&#xa0;V (vs.Ag/AgCl) was observed on SWCNT/POAP/SPCE for detection of NAD<sup>+</sup> using differential pulse voltammetry technique, which is non-enzymatic sensing. The sensitivity and limit of detection were 0.0618 µA/µM and 1.0 µM, respectively. A good linear relationship (<i>R</i><sup>2</sup> = 0.995) was established in the concentration range from 5.0 µM to 100 µM. Additionally, recoveries between 103.2% and 109.4% with RSD values much less than 3% were obtained. It revealed good efficiency of SWCNT/POAP/SPCE towards determination of NAD<sup>+</sup> in pig serum samples.</p> Graphical Abstract <p></p>

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A Disposable and Non-Enzymatic Sensor for Determination of Nicotinamide Adenine Dinucleotide in Pig Serum

  • 期刊论文

  • 发表日期：

    2026年5月18日

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  • Xiaoling He,
  • Guangzhong Li,
  • Sheng Ma,
  • Yanyao Chen,
  • Tian Ouyang,
  • Jiani Chen,
  • Jiyao Zhang,
  • Taoda Liu,
  • Dawei Pan,
  • Wenyan Tao,
  • Hong Wei

摘要

A disposable and non-enzymatic sensor had been constructed for determination of oxidized form of nicotinamide adenine dinucleotide (NAD+) in pig serum samples successfully. Single-walled carbon nanotubes (SWCNT) were immobilized on screen-printed carbon electrode (SPCE) by co-polymerized with monomer o-aminophenol (o-AP) (POAP) by cyclic voltammetry technique. Electrochemical impedance spectroscopy showed SWCNT/POAP/SPCE possesses a higher conductivity of 12.2 Ω⋅cm2, which is identified as an effective electrocatalyst for determining NAD+. A reduction peak at a potential of around −0.31 V (vs.Ag/AgCl) was observed on SWCNT/POAP/SPCE for detection of NAD+ using differential pulse voltammetry technique, which is non-enzymatic sensing. The sensitivity and limit of detection were 0.0618 µA/µM and 1.0 µM, respectively. A good linear relationship (R2 = 0.995) was established in the concentration range from 5.0 µM to 100 µM. Additionally, recoveries between 103.2% and 109.4% with RSD values much less than 3% were obtained. It revealed good efficiency of SWCNT/POAP/SPCE towards determination of NAD+ in pig serum samples.

Graphical Abstract