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<p>A highly efficient and reliable electroanalytical biosensor for the estimation of melatonin (ME) employing pretreated graphene nanosheet uniformly-wrapped Ru nanoparticles (PTGNS/RuNPs) nanocomposite rational designed oxidized polydiaminonaphthalene/taurine (OPDAN/TA) has been performed. The OPDAN/TA/PTGNS/RuNPs nanocomposite biosensor has been characterized with numerous techniques, such as X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS) and Fourier transform infrared spectroscopy (FTIR). The PTGNS/RuNPs structure has been uniformly dispersed and influentially utilized into the OPDAN/TA that improves nanocomposite performance toward the estimation of ME. The nanocomposite biosensor not only inherits the electrochemical active surface area of PTGNS/RuNPs, while appears an excellent conductivity and electrocatalytic performance of the oxidized conducting copolymer PDAN/TA. A great and excellent sensitive achievement is conducted with the biosensor that was electropolymerized in deep eutectic solvents (DES) to improve catalytic effectives to ME with high influential charges transport voltammograms, while eliminated any biological interfering species. Furthermore, the nanocomposite biosensor was then utilized in the estimation of ME with the wide linear range from 1.0–160&#xa0;μM, the limit of detection (LOD) and limit of quantitation (LOQ) of 0.015 ± 0.002&#xa0;µM and 0.050 ± 0.002&#xa0;µM with a sensitivity value of 4.2 μA μM<sup>–1</sup>. The reproducibility and repeatability of the biosensor were also examined with the relative standard deviation (RSD) of 3.23 and 3.62, respectively. The high selectivity and stability of the OPDAN/TA/PTGNS/RuNPs biosensor to ME estimation may be because of the functionality and surface nature of the OPDAN/TA that was completely dispersed on the PTGNS/RuNPs nanocomposite. Finally, the proposed nanocomposite biosensor was operated successfully and applied to the analytical examination of ME in biological fluids samples.</p> Graphical Abstract <p></p>

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Rational Designed Biosensor for Melatonin Estimation in Biological Fluids Using Graphene Nanosheet Uniformly-Wrapped Ru Nanoparticles Supported Polydiaminonaphthalene/Taurine Matrix

  • 期刊论文

  • 发表日期：

    2026年1月6日

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  • Adel A. Abdelwahab,
  • Salah Eid,
  • Ahmed H. Naggar,
  • A. M. Nassar,
  • Yoon-Bo Shim

摘要

A highly efficient and reliable electroanalytical biosensor for the estimation of melatonin (ME) employing pretreated graphene nanosheet uniformly-wrapped Ru nanoparticles (PTGNS/RuNPs) nanocomposite rational designed oxidized polydiaminonaphthalene/taurine (OPDAN/TA) has been performed. The OPDAN/TA/PTGNS/RuNPs nanocomposite biosensor has been characterized with numerous techniques, such as X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS) and Fourier transform infrared spectroscopy (FTIR). The PTGNS/RuNPs structure has been uniformly dispersed and influentially utilized into the OPDAN/TA that improves nanocomposite performance toward the estimation of ME. The nanocomposite biosensor not only inherits the electrochemical active surface area of PTGNS/RuNPs, while appears an excellent conductivity and electrocatalytic performance of the oxidized conducting copolymer PDAN/TA. A great and excellent sensitive achievement is conducted with the biosensor that was electropolymerized in deep eutectic solvents (DES) to improve catalytic effectives to ME with high influential charges transport voltammograms, while eliminated any biological interfering species. Furthermore, the nanocomposite biosensor was then utilized in the estimation of ME with the wide linear range from 1.0–160 μM, the limit of detection (LOD) and limit of quantitation (LOQ) of 0.015 ± 0.002 µM and 0.050 ± 0.002 µM with a sensitivity value of 4.2 μA μM–1. The reproducibility and repeatability of the biosensor were also examined with the relative standard deviation (RSD) of 3.23 and 3.62, respectively. The high selectivity and stability of the OPDAN/TA/PTGNS/RuNPs biosensor to ME estimation may be because of the functionality and surface nature of the OPDAN/TA that was completely dispersed on the PTGNS/RuNPs nanocomposite. Finally, the proposed nanocomposite biosensor was operated successfully and applied to the analytical examination of ME in biological fluids samples.

Graphical Abstract