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Background <p>Long noncoding RNAs (lncRNAs) are critical regulators of cancer progression, immune dynamics, and therapeutic response. <i>MAGI2-AS3</i> has been implicated in multiple malignancies; however, its pan-cancer relevance and specific role in colorectal cancer (CRC) remain incompletely understood.</p> Methods <p><i>MAGI2-AS3</i> expression was analyzed across cancers using bulk (TCGA, GTEx) and single-cell RNA-seq data. Functional enrichment, survival (Kaplan–Meier, Cox regression), diagnostic (ROC), immune infiltration (8 algorithms), immunotherapy response, drug sensitivity, and genomic/epigenetic analyses were performed. Expression was validated by RT-qPCR in 10 paired CRC tissues.</p> Results <p><i>MAGI2-AS3</i> expression exhibited marked heterogeneity across cancer types, with consistent downregulation in CRC and preferential enrichment in stromal and myeloid cell populations. Functional analyses linked <i>MAGI2-AS3</i> to epithelial–mesenchymal transition, immune regulation, and cytokine signaling. While prognostic associations were context dependent across cancers, CRC demonstrated a distinct pattern: <i>MAGI2-AS3</i> was consistently downregulated and showed robust diagnostic performance. Paradoxically, higher expression within tumors was associated with poorer survival outcomes and an inflamed yet immunosuppressive tumor microenvironment characterized by reduced response to immune checkpoint blockade, indicating a complex, compartment-specific role. Drug sensitivity and epigenetic analyses further underscored cancer-type–specific regulatory patterns, which were experimentally validated by RT-qPCR in CRC tissues.</p> Conclusion <p><i>MAGI2-AS3</i> functions as a context-dependent regulator with strong diagnostic relevance and complex prognostic implications in colorectal cancer. Its involvement in immune modulation, therapeutic response, and tumor progression highlights its potential as a biomarker for precision oncology, warranting further mechanistic and clinical investigation.</p>

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Pan-cancer and single-cell analysis identifies MAGI2-AS3 as an immune regulator and prognostic biomarker with a focus on colorectal cancer

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月17日

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  • Fatemeh Maghool,
  • Maedeh Mirzamohammadi,
  • Seyed Ali Taheri Hatkehlouei,
  • Samane Mohammadzadeh,
  • Aida Heidari,
  • Mohammad Hassan Emami,
  • Pouria Samadi

摘要

Background

Long noncoding RNAs (lncRNAs) are critical regulators of cancer progression, immune dynamics, and therapeutic response. MAGI2-AS3 has been implicated in multiple malignancies; however, its pan-cancer relevance and specific role in colorectal cancer (CRC) remain incompletely understood.

Methods

MAGI2-AS3 expression was analyzed across cancers using bulk (TCGA, GTEx) and single-cell RNA-seq data. Functional enrichment, survival (Kaplan–Meier, Cox regression), diagnostic (ROC), immune infiltration (8 algorithms), immunotherapy response, drug sensitivity, and genomic/epigenetic analyses were performed. Expression was validated by RT-qPCR in 10 paired CRC tissues.

Results

MAGI2-AS3 expression exhibited marked heterogeneity across cancer types, with consistent downregulation in CRC and preferential enrichment in stromal and myeloid cell populations. Functional analyses linked MAGI2-AS3 to epithelial–mesenchymal transition, immune regulation, and cytokine signaling. While prognostic associations were context dependent across cancers, CRC demonstrated a distinct pattern: MAGI2-AS3 was consistently downregulated and showed robust diagnostic performance. Paradoxically, higher expression within tumors was associated with poorer survival outcomes and an inflamed yet immunosuppressive tumor microenvironment characterized by reduced response to immune checkpoint blockade, indicating a complex, compartment-specific role. Drug sensitivity and epigenetic analyses further underscored cancer-type–specific regulatory patterns, which were experimentally validated by RT-qPCR in CRC tissues.

Conclusion

MAGI2-AS3 functions as a context-dependent regulator with strong diagnostic relevance and complex prognostic implications in colorectal cancer. Its involvement in immune modulation, therapeutic response, and tumor progression highlights its potential as a biomarker for precision oncology, warranting further mechanistic and clinical investigation.