Background <p>Cervical cancer represents one of the most common malignant tumors affecting women worldwide with unsatisfactory therapeutic outcome. CD24 is well acknowledged that could express on cancer cell and block the phagocytosis of macrophages in tumor microenvironment. However, it is unclear whether the cervical cancer derived extracellular vesicles (CC-EVs) block the phagocytosis of macrophages.</p> Methods <p>CD24 expression was detected in cervical cancer tissues. The CC-EVs were isolated to treat the macrophages. Furthermore, the CD24 expression was detected in CC-EVs. Then we isolated CD24-EVs through transient transfection and CD24^KO-EVs through CRISPR/Cas9, to treat the macrophages. The phagocytic ability and phenotype of macrophages were detected accordingly.</p> Results <p>Cervical cancer derived EVs could decrease the phagocytosis of macrophages. The CC-EVs expressed high level of CD24, which play the key role in blocking the phagocytosis of macrophages.</p> Conclusions <p>The cervical cancer derived EVs express higher level of CD24, which inhibits the phagocytosis of macrophages.</p>