Scholarlink

Background <p>Epigallocatechin gallate (EGCG) is a catechin, a type of flavonoid that is found in high concentrations in green tea. EGCG has been demonstrated to exhibit anti-proliferative, anti-angiogenic, and pro-apoptotic effects across various cancer cell lines. Furthermore, research indicates that EGCG plays a significant role in the antiproliferation and apoptosis of human nasopharyngeal carcinoma (NPC) cells. The anticancer activity of EGCG is mediated through its interference with multiple hallmarks of cancer; however, the specific targets and mechanisms by which EGCG combats NPC remain unclear.</p> Methods <p>This study integrated cell line experiments, network pharmacology, molecular docking, and dynamics simulations to elucidate EGCG’s therapeutic mechanisms in NPC and identify potential targets, while scRNA-seq and MR analyses assessed key target gene-NPC relationships.</p> Results <p>Analysis of the GEO database and RNA sequencing of EGCG-treated NPC (HK-1) cells identified 165 potential targets associated with both EGCG and NPC. Network analysis revealed ten core targets, with EIF2AK2, IFIT1, and STAT1 being key. KEGG and GO analyses highlighted important pathways, including PI3K-Akt, phospholipase D signaling, and EBV infection. Molecular docking showed strong interactions between EGCG and the core targets, and molecular dynamics simulations confirmed complex stability. Finally, differential expression profiling of key target genes at the single-cell level demonstrated a concordance with transcriptomic data, while MR analyses validated the causal relationships between these target genes and the risk of NPC.</p> Conclusion <p>These findings offer a theoretical basis for the molecular mechanisms underlying EGCG’s therapeutic effects on NPC.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Elucidating EGCG’ s targets in nasopharyngeal carcinoma through combined cell-based assays and transcriptomic sequencing

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月20日

查看全文
  • Yuhang Yang,
  • Guangxu Xuan,
  • Zhang Feng,
  • Ming Deng,
  • Fangxian Liu,
  • Qianghe Liu,
  • Wenhua Wang,
  • Zifang Li,
  • Dongzhi Zuo,
  • Zhenya Li,
  • Congbao Wei,
  • Wenqi Luo,
  • Fei Li,
  • Ning Ma,
  • Feng He,
  • Mariko Murata

摘要

Background

Epigallocatechin gallate (EGCG) is a catechin, a type of flavonoid that is found in high concentrations in green tea. EGCG has been demonstrated to exhibit anti-proliferative, anti-angiogenic, and pro-apoptotic effects across various cancer cell lines. Furthermore, research indicates that EGCG plays a significant role in the antiproliferation and apoptosis of human nasopharyngeal carcinoma (NPC) cells. The anticancer activity of EGCG is mediated through its interference with multiple hallmarks of cancer; however, the specific targets and mechanisms by which EGCG combats NPC remain unclear.

Methods

This study integrated cell line experiments, network pharmacology, molecular docking, and dynamics simulations to elucidate EGCG’s therapeutic mechanisms in NPC and identify potential targets, while scRNA-seq and MR analyses assessed key target gene-NPC relationships.

Results

Analysis of the GEO database and RNA sequencing of EGCG-treated NPC (HK-1) cells identified 165 potential targets associated with both EGCG and NPC. Network analysis revealed ten core targets, with EIF2AK2, IFIT1, and STAT1 being key. KEGG and GO analyses highlighted important pathways, including PI3K-Akt, phospholipase D signaling, and EBV infection. Molecular docking showed strong interactions between EGCG and the core targets, and molecular dynamics simulations confirmed complex stability. Finally, differential expression profiling of key target genes at the single-cell level demonstrated a concordance with transcriptomic data, while MR analyses validated the causal relationships between these target genes and the risk of NPC.

Conclusion

These findings offer a theoretical basis for the molecular mechanisms underlying EGCG’s therapeutic effects on NPC.