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Purpose <p>We aimed to evaluate the potential effects of Plantago major (PM) to enhance the radiosensitivity of human breast adenocarcinoma cell line (MCF-7) in an animal model.</p> Materials and methods <p>Seventy-two female Balb/c mice were divided into nine groups (8 mice per group) as follows: MCF-7 breast cancer control group, MCF-7+low dose of PM (1000&#xa0;mg/kg), MCF-7+high dose of PM (1500&#xa0;mg/kg), MCF-7+3&#xa0;Gy superficial X-ray, MCF-7+5&#xa0;Gy superficial X-ray, MCF-7+1000&amp;1500&#xa0;mg/kg of PM with 3 and 5&#xa0;Gy irradiations. For each treatment group, micronuclei in 500 binucleate MCF-7 cells from a minimum of three experiments were counted. The alkaline comet assay was used to calculate % DNA in the tail and % of apoptotic comets. The tumor size in the treated groups (3 mice per group) was assessed at 4- and 8-weeks post-treatment.</p> Results <p>The number of total micronuclei and binucleated micronuclei in the PM and/or irradiation treated groups was significantly higher than in the control group (P &lt; 0.05). Irradiation+PM resulted in a significant increase treatment effect compared to the irradiation-only groups (P &lt; 0.01). In addition, the higher PM concentration had a significantly higher number of micronucleated binucleate cells when combined with 5&#xa0;Gy irradiation (P = 0.022). Irradiation alone or in combination with PM resulted in significant increases in percentages of DNA in tail and apoptotic comet values compared to the PM-only treatment groups (P &lt; 0.02). Combining 5&#xa0;Gy of irradiation with 1000&#xa0;mg/kg of PM led to a 26% reduction in tumor size (0.28±0.04 vs. 0.38 ± 0.03) after 4&#xa0;weeks, and combining 5&#xa0;Gy of irradiation with 1500&#xa0;mg/kg of PM resulted in a 40% decrease in tumor volume after 4&#xa0;weeks (0.21±0.04 vs. 0.35±0.03).</p> Conclusion <p>PM extract at both doses demonstrated an antitumor effect on induced MCF-7 breast cancer tumors, with this effect being enhanced when combined with irradiation.</p>

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Enhancement of radiation effects by Plantago major in MCF-7 human breast cancer: an animal study

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月14日

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  • Hongliang Zhao,
  • Hongxiang Gao,
  • Jing Kang,
  • Jiexin Chen,
  • Huiling Ouyang,
  • Peiyao Chen,
  • Hussain Shaik Althaf,
  • Turki Mayudh Alrubie,
  • Maddu Narendra,
  • Kai Wang

摘要

Purpose

We aimed to evaluate the potential effects of Plantago major (PM) to enhance the radiosensitivity of human breast adenocarcinoma cell line (MCF-7) in an animal model.

Materials and methods

Seventy-two female Balb/c mice were divided into nine groups (8 mice per group) as follows: MCF-7 breast cancer control group, MCF-7+low dose of PM (1000 mg/kg), MCF-7+high dose of PM (1500 mg/kg), MCF-7+3 Gy superficial X-ray, MCF-7+5 Gy superficial X-ray, MCF-7+1000&1500 mg/kg of PM with 3 and 5 Gy irradiations. For each treatment group, micronuclei in 500 binucleate MCF-7 cells from a minimum of three experiments were counted. The alkaline comet assay was used to calculate % DNA in the tail and % of apoptotic comets. The tumor size in the treated groups (3 mice per group) was assessed at 4- and 8-weeks post-treatment.

Results

The number of total micronuclei and binucleated micronuclei in the PM and/or irradiation treated groups was significantly higher than in the control group (P < 0.05). Irradiation+PM resulted in a significant increase treatment effect compared to the irradiation-only groups (P < 0.01). In addition, the higher PM concentration had a significantly higher number of micronucleated binucleate cells when combined with 5 Gy irradiation (P = 0.022). Irradiation alone or in combination with PM resulted in significant increases in percentages of DNA in tail and apoptotic comet values compared to the PM-only treatment groups (P < 0.02). Combining 5 Gy of irradiation with 1000 mg/kg of PM led to a 26% reduction in tumor size (0.28±0.04 vs. 0.38 ± 0.03) after 4 weeks, and combining 5 Gy of irradiation with 1500 mg/kg of PM resulted in a 40% decrease in tumor volume after 4 weeks (0.21±0.04 vs. 0.35±0.03).

Conclusion

PM extract at both doses demonstrated an antitumor effect on induced MCF-7 breast cancer tumors, with this effect being enhanced when combined with irradiation.