Objectives <p>This study presents the first psychometric validation of the Mindful Self-Care Scale (MSCS-Standard) in Russian-speaking populations, addressing a critical gap in culturally validated mindfulness assessment tools.</p> Methods <p>Following forward–backward translation procedures and cognitive pilot testing, the 33-item Russian MSCS was administered to 600 Russian adults (39% male;&#xa0;<i>M</i>&#xa0;age = 41.4,&#xa0;<i>SD</i> = 12.0). Psychometric evaluation included item analysis, exploratory and confirmatory factor analyses, measurement invariance testing, and convergent validity assessment.</p> Results <p>The Russian MSCS demonstrated excellent internal consistency (Cronbach’s&#xa0;<i>α</i> = 0.92) with acceptable hierarchical omega (McDonald’s&#xa0;<i>ωₕ</i> = 0.74) and subscale reliabilities from 0.70 to 0.83. Exploratory factor analysis revealed a six-factor structure accounting for 63% of the total variance. Confirmatory factor analysis supported the six-factor structure with excellent fit (comparative fit index = 0.97, Tucker-Lewis index = 0.97, root mean square error of approximation = 0.07, standardized root mean square residual = 0.07). Scalar measurement invariance was confirmed across gender and age groups. Convergent validity was established through correlations with self-compassion (<i>r</i> = 0.34) and psychological well-being (<i>r</i> = 0.18).</p> Conclusions <p>The Russian MSCS-Standard exhibits strong reliability and validity evidence comparable to other cross-cultural adaptations, enabling systematic investigation of mindful self-care practices in Russian-speaking contexts and facilitating international comparative research.</p> Preregistration <p>This study was not preregistered.</p>