Objectives <p>Recent years have witnessed a marked proliferation in the interest in and relevant literature pertaining to the holistic benefits experienced from the practice of mindfulness. Although mindfulness-based programs are recognized for clinical and non-clinical populations, little attention is dedicated to building mindfulness education into the core curriculum of university students. This novel investigation aimed to examine the efficacy of a holistic mindful-based health promotion curriculum embedded in a required course in fostering mindfulness, healthy lifestyle choices, stress management, lower perceived anxiety, improved resilience, and greater relationship stability.</p> Method <p>Forty-seven (<i>n</i> = 47) volunteer participant students from five different sections of a required university course completed a demographic questionnaire and pre- and post-course self-report outcome measures including Five Facet Mindfulness Questionnaire (FFMQ), Health-Promoting Lifestyle Profile-II (HPLP-II), Perceived Stress Scale (PSS), Beck Anxiety Inventory (BAI), The Resilience Scale (RS), and Relationship Scales Questionnaire (RSQ).</p> Results <p>The study findings indicated that mindfulness, as the foundation of a holistic health promotion course curriculum, can significantly improve (1) the ability to be mindful and aware, (2) healthy lifestyle choices, (3) stress management, (4) perceived anxiety, (5) resilience, and (6) feelings of security in relationships.</p> Conclusions <p>These findings underscore the need for thoughtful consideration of mindfulness-based practices in university course curriculum to foster holistic health and well-being in this vulnerable population. Limitations of the current study and future research considerations are discussed.</p> Preregistration <p>This study was not preregistered.</p>