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<p>This review examines the applications of indirect lateral flow assays (ILFAs) and indirect ELISA-based LFAs as robust, paper-based diagnostic platforms for the identification of contaminants in food products. ILFAs employ antibodies, aptamers, and their hybrid composites combined with molecularly imprinted polymers to deliver a cost-effective diagnostic tool. Capable of detecting biological and chemical adulterants within 3 to 30&#xa0;min, these assays enable efficient identification directly at the point-of-care. The operational principles, the functions of different components, and various assay formats of ILFAs are elaborated, with particular focus on their suitability for monitoring complex food matrices. This review highlights recent progress in ILFA technology, focusing on innovative signal amplification methods and novel labeling techniques designed to enhance sensitivity and reproducibility in food safety testing. Continued developments in ILFA offer significant potential to improve diagnostic accuracy and accessibility within the food industry, thereby supporting stronger regulatory compliance and advancing public health protection.</p> Graphical Abstract <p></p>

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From Indirect ELISA to Advanced Lateral Flow Assays: Advances and Applications in Food Quality and Safety Monitoring

  • 期刊论文

  • 发表日期：

    2026年7月16日

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  • Sepideh Hassibian,
  • Masoomeh Esmaelpourfarkhani,
  • Mona Alibolandi,
  • Mohammad Ramezani,
  • Seyed Mohammad Taghdisi,
  • Seyed Mohsen Dehnavi,
  • Khalil Abnous

摘要

This review examines the applications of indirect lateral flow assays (ILFAs) and indirect ELISA-based LFAs as robust, paper-based diagnostic platforms for the identification of contaminants in food products. ILFAs employ antibodies, aptamers, and their hybrid composites combined with molecularly imprinted polymers to deliver a cost-effective diagnostic tool. Capable of detecting biological and chemical adulterants within 3 to 30 min, these assays enable efficient identification directly at the point-of-care. The operational principles, the functions of different components, and various assay formats of ILFAs are elaborated, with particular focus on their suitability for monitoring complex food matrices. This review highlights recent progress in ILFA technology, focusing on innovative signal amplification methods and novel labeling techniques designed to enhance sensitivity and reproducibility in food safety testing. Continued developments in ILFA offer significant potential to improve diagnostic accuracy and accessibility within the food industry, thereby supporting stronger regulatory compliance and advancing public health protection.

Graphical Abstract