Scholarlink

<p>Biofilms are in many sectors a persistent technical challenge that can lead to reduced efficiency in industrial processes, increased maintenance costs, and potential product contamination. Furthermore, biofilms can contribute to the development of antimicrobial resistance, posing health risks in medical and food production environments, as well as reducing the effectiveness of cleaning and sterilization efforts. Therefore, we investigated the impact of antimicrobial silver nanoparticles (AgNPs) on macroscopically visible biofilms of <i>Cupriavidus metallidurans</i> CH34, a metal-resistant and oligotrophic bacterium. We accelerated the time-consuming process of naturally forming macroscopic biofilms via a laboratory setup using porous synthetic surfaces. This setup simulates growth dynamics on air-exposed and nutrient-leaking substrates such as rocks, wood, polymeric coatings, joints and dripping pipes. Remarkably, 24-hour matured biofilms were unaffected by AgNPs at concentrations as high as 750&#xa0;mg/L. Biofilm resilience to AgNPs was age-dependent and although densely populated, young biofilms (6–9&#xa0;h) were inactivated by AgNPs, older ones (14–24&#xa0;h) exhibited tolerance to the same conditions. The increased tolerance in older biofilms was not attributed to cell density, but potentially to other factors like extracellular polymeric substances (EPS) or their metabolic state. AgNPs induced oxidative stress in exposed biofilms but did not result in microscopically observable morphological changes in the biofilm-resident cells, indicating that inhibition mechanisms did not involve outer membrane disruption. The proteomic analysis of macroscopic biofilms exposed to AgNPs revealed an age-independent protein set that was consistently upregulated/down regulated across all analyzed biofilm stages as well as age-specific pathways. These findings suggest complex adaptive molecular mechanisms in <i>C. metallidurans</i> biofilms that confer tolerance to AgNPs, involving oxidative stress management, iron metabolism, and electron transport chain adjustments.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The Dynamic Response of Cupriavidus Metallidurans CH34 Cells and Biofilms to Silver Nanoparticles and the Critical Role of Biofilm maturation

  • 期刊论文

  • 发表日期：

    2026年7月1日

查看全文
  • Nissem Abdeljelil,
  • Timothej Patocka,
  • Luna Hendrickx,
  • Najla Ben Miloud Yahia,
  • Surya Gupta,
  • Felice Mastroleo,
  • Abdelwaheb Chatti,
  • Ruddy Wattiez,
  • David Gillan,
  • Rob Van Houdt

摘要

Biofilms are in many sectors a persistent technical challenge that can lead to reduced efficiency in industrial processes, increased maintenance costs, and potential product contamination. Furthermore, biofilms can contribute to the development of antimicrobial resistance, posing health risks in medical and food production environments, as well as reducing the effectiveness of cleaning and sterilization efforts. Therefore, we investigated the impact of antimicrobial silver nanoparticles (AgNPs) on macroscopically visible biofilms of Cupriavidus metallidurans CH34, a metal-resistant and oligotrophic bacterium. We accelerated the time-consuming process of naturally forming macroscopic biofilms via a laboratory setup using porous synthetic surfaces. This setup simulates growth dynamics on air-exposed and nutrient-leaking substrates such as rocks, wood, polymeric coatings, joints and dripping pipes. Remarkably, 24-hour matured biofilms were unaffected by AgNPs at concentrations as high as 750 mg/L. Biofilm resilience to AgNPs was age-dependent and although densely populated, young biofilms (6–9 h) were inactivated by AgNPs, older ones (14–24 h) exhibited tolerance to the same conditions. The increased tolerance in older biofilms was not attributed to cell density, but potentially to other factors like extracellular polymeric substances (EPS) or their metabolic state. AgNPs induced oxidative stress in exposed biofilms but did not result in microscopically observable morphological changes in the biofilm-resident cells, indicating that inhibition mechanisms did not involve outer membrane disruption. The proteomic analysis of macroscopic biofilms exposed to AgNPs revealed an age-independent protein set that was consistently upregulated/down regulated across all analyzed biofilm stages as well as age-specific pathways. These findings suggest complex adaptive molecular mechanisms in C. metallidurans biofilms that confer tolerance to AgNPs, involving oxidative stress management, iron metabolism, and electron transport chain adjustments.