Scholarlink

<p>Selenium (Se) is an essential micronutrient with critical biological roles, yet its narrow margin between nutritional requirement and toxicity limits conventional supplementation. Selenium nanoparticles, remarkably anisotropic forms, offer improved bioactivity and reduced toxicity. This study reports the extracellular biosynthesis of selenium nanorods (SeNRs) by the endophytic fungus <i>Alternaria longipes</i>, isolated from <i>Podophyllum hexandrum</i>. The fungal biomass reduced sodium selenite (Na<sub>2</sub>SeO<sub>3</sub>) to elemental Se under optimized conditions (5.0 mM Na<sub>2</sub>SeO<sub>3</sub>, pH 9.0, Sabouraud Dextrose Broth) within 4 days, yielding predominantly rod-shaped particles (250–600&#xa0;nm). UV-Vis spectroscopy, FTIR, XRD, and FE-SEM characterisation confirmed Se⁰ crystallinity and nanorod morphology. Biosynthesis efficiency was strongly influenced by selenite concentration, pH, and nutrient composition, with glucose and peptone as optimal carbon and nitrogen sources. Antioxidant assays revealed moderate radical scavenging (IC<sub>50</sub> = 65.79&#xa0;µg/mL) and reducing power (IC<sub>50</sub> = 72.28&#xa0;µg/mL), while cytotoxicity testing against MCF-7 breast cancer cells showed notable activity (IC<sub>50</sub> = 25.31&#xa0;µg/mL), suggesting both redox-mediated and shape-dependent effects. These findings establish <i>A. longipes</i> as a promising biofactory for eco-friendly SeNR production, offering potential nanomedicine, agriculture, and environmental remediation applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biogenic Production of Selenium Nanorods by the Endophytic Fungus Alternaria Longipes and Evaluation of their Antioxidant and Cytotoxic Properties

  • 期刊论文

  • 发表日期：

    2026年5月23日

查看全文
  • Anviksha Anviksha,
  • M. Sudhakara Reddy

摘要

Selenium (Se) is an essential micronutrient with critical biological roles, yet its narrow margin between nutritional requirement and toxicity limits conventional supplementation. Selenium nanoparticles, remarkably anisotropic forms, offer improved bioactivity and reduced toxicity. This study reports the extracellular biosynthesis of selenium nanorods (SeNRs) by the endophytic fungus Alternaria longipes, isolated from Podophyllum hexandrum. The fungal biomass reduced sodium selenite (Na2SeO3) to elemental Se under optimized conditions (5.0 mM Na2SeO3, pH 9.0, Sabouraud Dextrose Broth) within 4 days, yielding predominantly rod-shaped particles (250–600 nm). UV-Vis spectroscopy, FTIR, XRD, and FE-SEM characterisation confirmed Se⁰ crystallinity and nanorod morphology. Biosynthesis efficiency was strongly influenced by selenite concentration, pH, and nutrient composition, with glucose and peptone as optimal carbon and nitrogen sources. Antioxidant assays revealed moderate radical scavenging (IC50 = 65.79 µg/mL) and reducing power (IC50 = 72.28 µg/mL), while cytotoxicity testing against MCF-7 breast cancer cells showed notable activity (IC50 = 25.31 µg/mL), suggesting both redox-mediated and shape-dependent effects. These findings establish A. longipes as a promising biofactory for eco-friendly SeNR production, offering potential nanomedicine, agriculture, and environmental remediation applications.