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<p>Neurodegenerative disorders are closely associated with oxidative stress, neuroinflammation, and disrupted neuronal homeostasis, demanding the development of multifunctional and sustained therapeutic strategies. In this study, Sahaj-vati, a classical Ayurvedic polyherbal formulation comprising Shilajeet, Guggul, Chitrak, Haridra, and Agnimantha was extracted using Soxhlet-assisted methanolic extraction and subsequently modified into electrospun nanofibers (SNFs) to enhance its stability, bioactivity, and translational applicability. The extract exhibited total phenolic and flavonoid contents of 0.85&#xa0;mg GAE/g DW and 2.497&#xa0;mg QE/g DW, respectively. The gas chromatography-mass spectrometry (GC-MS) analysis confirmed the presence of bioactive phytoconstituents such as tumerone, caffeine, theobromine, and oleic acid, associated with antioxidant and neuroprotective functions. The morphological and physicochemical characterization revealed uniform nanofibrous architecture with an average diameter of ~ 559&#xa0;nm and thermal stability up to 350&#xa0;°C. The biological evaluation demonstrated significantly enhanced antioxidant activity of SNFs, with DPPH and FRAP IC<sub>50</sub> values of 27.09&#xa0;µg/mL and 34.89&#xa0;µg/mL, respectively, compared to the crude extract. The SNFs also showed improved anti-inflammatory activity (IC<sub>50</sub> = 34.89&#xa0;µg/mL) and moderate acetylcholinesterase inhibition (IC<sub>50</sub> = 60.73&#xa0;µg/mL). The cytocompatibility assays on L929 and SH-SY5Y cell lines confirmed high cell viability (&gt; 85–90%), indicating excellent biocompatibility. Furthermore, Franz diffusion studies revealed rapid and efficient phytochemical release (~ 80% within 60&#xa0;min), demonstrating superior permeation characteristics. Overall, Sahaj-vati-loaded electrospun nanofibers represent a promising multifunctional platform for delivering neuroprotective phytoconstituents, effectively bridging traditional Ayurvedic medicine with advanced nanotechnology for biomedical applications.</p>

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Synthesis and Evaluation of Sahaj-vati-loaded Electrospun Nanofibers As a Multifunctional Phytotherapeutic Delivery System: An Integrative Ayurvedic-Nanobiotechnology Approach

  • 期刊论文

  • 发表日期：

    2026年5月21日

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  • Mansi M. Jadhav,
  • Arati S. Kulkarni,
  • Shivtej M. Mane,
  • Prafull B. Dandge,
  • Nisha A. Nerlekar,
  • Sandip S. Kale,
  • Tukaram D. Dongale,
  • Padma B. Dandge

摘要

Neurodegenerative disorders are closely associated with oxidative stress, neuroinflammation, and disrupted neuronal homeostasis, demanding the development of multifunctional and sustained therapeutic strategies. In this study, Sahaj-vati, a classical Ayurvedic polyherbal formulation comprising Shilajeet, Guggul, Chitrak, Haridra, and Agnimantha was extracted using Soxhlet-assisted methanolic extraction and subsequently modified into electrospun nanofibers (SNFs) to enhance its stability, bioactivity, and translational applicability. The extract exhibited total phenolic and flavonoid contents of 0.85 mg GAE/g DW and 2.497 mg QE/g DW, respectively. The gas chromatography-mass spectrometry (GC-MS) analysis confirmed the presence of bioactive phytoconstituents such as tumerone, caffeine, theobromine, and oleic acid, associated with antioxidant and neuroprotective functions. The morphological and physicochemical characterization revealed uniform nanofibrous architecture with an average diameter of ~ 559 nm and thermal stability up to 350 °C. The biological evaluation demonstrated significantly enhanced antioxidant activity of SNFs, with DPPH and FRAP IC50 values of 27.09 µg/mL and 34.89 µg/mL, respectively, compared to the crude extract. The SNFs also showed improved anti-inflammatory activity (IC50 = 34.89 µg/mL) and moderate acetylcholinesterase inhibition (IC50 = 60.73 µg/mL). The cytocompatibility assays on L929 and SH-SY5Y cell lines confirmed high cell viability (> 85–90%), indicating excellent biocompatibility. Furthermore, Franz diffusion studies revealed rapid and efficient phytochemical release (~ 80% within 60 min), demonstrating superior permeation characteristics. Overall, Sahaj-vati-loaded electrospun nanofibers represent a promising multifunctional platform for delivering neuroprotective phytoconstituents, effectively bridging traditional Ayurvedic medicine with advanced nanotechnology for biomedical applications.