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<p>Fucoidan, a sulphated polysaccharide derived from <i>Sargassum wightii</i>, holds significant therapeutic potential due to its antioxidant, antimicrobial, and wound-healing properties. Despite its therapeutic properties, its clinical efficacy is limited by poor bioavailability and instability. This study reports the successful encapsulation of fucoidan in liposomes employing the thin-film hydration technique, followed by chitosan coating to enhance its stability and biological activity. Structural integrity and successful encapsulation were confirmed through FTIR and UV–Vis spectroscopy. Antioxidant activity assessed via DPPH and hydrogen peroxide scavenging assays demonstrated concentration-dependent radical scavenging, with chitosan-coated formulations exhibiting superior efficacy. The formulation was reported to exhibit strong antioxidant potential, as indicated by DPPH (38.65% at 500&#xa0;μg/ml) and H₂O₂ (40.707% at 400 μg/ml). Antimicrobial testing revealed notable activity against the Gram-negative bacterium <i>Escherichia coli</i>, but not against the Gram-positive bacterium <i>Bacillus subtilis</i>, suggesting a narrow-spectrum antibacterial potential. The antimicrobial assays conducted reported a zone of inhibition of 11.4&#xa0;mm for a concentration of 2&#xa0;mg/ml. Furthermore, scratch wound assays and MTT-based cytotoxicity analysis on L929 fibroblast cells indicated promising wound-healing activity, with a wound closure of 92.72% observed 72&#xa0;h after treatment with the sample. The IC₅₀ value of 100&#xa0;μg/ml was also reported to have high cell viability of 84.22%. These findings underscore the potential of chitosan-coated liposomal fucoidan as a multifunctional bioactive system for pharmaceutical and biomedical applications.</p> Graphical Abstract <p></p>

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Chitosan Coated-Liposomal Microencapsulation of Fucoidan Extracted from Marine Sargassum wightii: Assessment of Its Biofunctional and Therapeutic Properties

  • 期刊论文

  • 发表日期：

    2026年5月8日

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  • Gicy Mathew,
  • Amruth P

摘要

Fucoidan, a sulphated polysaccharide derived from Sargassum wightii, holds significant therapeutic potential due to its antioxidant, antimicrobial, and wound-healing properties. Despite its therapeutic properties, its clinical efficacy is limited by poor bioavailability and instability. This study reports the successful encapsulation of fucoidan in liposomes employing the thin-film hydration technique, followed by chitosan coating to enhance its stability and biological activity. Structural integrity and successful encapsulation were confirmed through FTIR and UV–Vis spectroscopy. Antioxidant activity assessed via DPPH and hydrogen peroxide scavenging assays demonstrated concentration-dependent radical scavenging, with chitosan-coated formulations exhibiting superior efficacy. The formulation was reported to exhibit strong antioxidant potential, as indicated by DPPH (38.65% at 500 μg/ml) and H₂O₂ (40.707% at 400 μg/ml). Antimicrobial testing revealed notable activity against the Gram-negative bacterium Escherichia coli, but not against the Gram-positive bacterium Bacillus subtilis, suggesting a narrow-spectrum antibacterial potential. The antimicrobial assays conducted reported a zone of inhibition of 11.4 mm for a concentration of 2 mg/ml. Furthermore, scratch wound assays and MTT-based cytotoxicity analysis on L929 fibroblast cells indicated promising wound-healing activity, with a wound closure of 92.72% observed 72 h after treatment with the sample. The IC₅₀ value of 100 μg/ml was also reported to have high cell viability of 84.22%. These findings underscore the potential of chitosan-coated liposomal fucoidan as a multifunctional bioactive system for pharmaceutical and biomedical applications.

Graphical Abstract