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<p>Pathogenic microorganisms have become a serious threat to human beings all over the world as they cause severe disease illnesses. This study used supercritical carbon dioxide as a green solvent to prepare a nanocomposite composed of copper oxide (CuO) nanoparticles dispersed on the surface of reduced graphene oxide (rGO). The resulting nanocomposite was examined using a various of alanytical techniques. The developed CuO/rGO nanocomposite exhibited potenail antimicrobial ability against a food pathogenic bacterium, <i>Bacillus cereus</i>. Additionality, it showed high toxicity towards <i>B. cereus</i>, confirmed by the means of fluorescent live-dead counting of cells. Its mechanistic role against a food meneac was confirmed by the means of time-kill ability (complete inhibition at 200&#xa0;min), cell membrane integrity (OD of control: 1.62; CuO/rGO: 3.78 at 60&#xa0;min), membrane uptake (Control: 27.21%; Cuo/rGO: 64.33%), and membrane potential ability (Control: 58.33; CuO/rGO: 24.12) towards <i>B. cereus</i>. Study of scanning electron microscopy analyse resulted in the membrane disruption of <i>B. cereus</i> by the nanocomposite. Moreover, the CuO/rGO nanocomposite inhibited in vitro biofilm formation ability (Crystal violet uptake - Control: 31.33%; CuO/rGO: 59.11%) of <i>B. cereus</i>. Furthermore, the nanocomposite coating was used as a rinse solution for rice bowl packages. Interestingly, a rinsing solution (20% and 40–60&#xa0;min) significantly inhibited the CFU count of <i>B. cereus</i> in rice by 2.1 log cfu/g as compaered to control 6.2 log cfu/g. The outcomes highlight the effectiveness of nanocomposite coating against food menace <i>B. cereus</i>, suggesting that the developed nanocomposite could be applied as an effective antimicrobial marinade and/or a rinse for raw rice preservation aginst hazardous foodborne pathogens.</p>

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Biogenic Copper Oxide@rGO Nanocomposite for Decontamination of a Food Threat B. Cereus in a Rice Model

  • 期刊论文

  • 发表日期：

    2026年4月24日

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  • Mercyland Pamshong,
  • Ibansaralang Kharthangmaw,
  • Janita Saji,
  • Vikas Dubey,
  • Amer M. Alanazi,
  • Azmat Ali Khan,
  • Yuvaraj Haldorai,
  • Shruti Shukla

摘要

Pathogenic microorganisms have become a serious threat to human beings all over the world as they cause severe disease illnesses. This study used supercritical carbon dioxide as a green solvent to prepare a nanocomposite composed of copper oxide (CuO) nanoparticles dispersed on the surface of reduced graphene oxide (rGO). The resulting nanocomposite was examined using a various of alanytical techniques. The developed CuO/rGO nanocomposite exhibited potenail antimicrobial ability against a food pathogenic bacterium, Bacillus cereus. Additionality, it showed high toxicity towards B. cereus, confirmed by the means of fluorescent live-dead counting of cells. Its mechanistic role against a food meneac was confirmed by the means of time-kill ability (complete inhibition at 200 min), cell membrane integrity (OD of control: 1.62; CuO/rGO: 3.78 at 60 min), membrane uptake (Control: 27.21%; Cuo/rGO: 64.33%), and membrane potential ability (Control: 58.33; CuO/rGO: 24.12) towards B. cereus. Study of scanning electron microscopy analyse resulted in the membrane disruption of B. cereus by the nanocomposite. Moreover, the CuO/rGO nanocomposite inhibited in vitro biofilm formation ability (Crystal violet uptake - Control: 31.33%; CuO/rGO: 59.11%) of B. cereus. Furthermore, the nanocomposite coating was used as a rinse solution for rice bowl packages. Interestingly, a rinsing solution (20% and 40–60 min) significantly inhibited the CFU count of B. cereus in rice by 2.1 log cfu/g as compaered to control 6.2 log cfu/g. The outcomes highlight the effectiveness of nanocomposite coating against food menace B. cereus, suggesting that the developed nanocomposite could be applied as an effective antimicrobial marinade and/or a rinse for raw rice preservation aginst hazardous foodborne pathogens.