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<p>The rational design of biocompatible and durable tissue-engineered devices hinges on a detailed understanding of their interactions with surrounding cells and tissues. In this study, we evaluated the behavior of EA.hy926 endothelial cells and human gingival fibroblasts in contact with electrospun scaffolds fabricated from protein-enriched polyurethane. The influence of the scaffolds’ chemical composition, architecture, and surface physicochemical properties on cell behavior was assessed. Comprehensive analysis using scanning electron microscopy, real-time PCR, and endothelial cell viability assays revealed that cell-scaffold interactions are predominantly governed by the surface properties of the fibers. We established that the relative concentration of proteins (gelatin, albumin, and their mixtures) is a key factor controlling fiber surface modification and, consequently, cell-material interactions. This finding highlights the potential for optimizing scaffold properties by fine-tuning protein ratios to direct biological responses for specific clinical applications.</p>

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Cell Responses to Electrospun protein-enriched Polyurethane Scaffolds: Relative Contributions of Physicochemical and Morphological Cues

  • 期刊论文

  • 发表日期：

    2026年2月16日

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  • Vera Chernonosova,
  • Yu Wenhui,
  • Ekaterina Skorokhodova,
  • Victor Golyshev,
  • Aleksei Shefer,
  • Pavel Laktionov

摘要

The rational design of biocompatible and durable tissue-engineered devices hinges on a detailed understanding of their interactions with surrounding cells and tissues. In this study, we evaluated the behavior of EA.hy926 endothelial cells and human gingival fibroblasts in contact with electrospun scaffolds fabricated from protein-enriched polyurethane. The influence of the scaffolds’ chemical composition, architecture, and surface physicochemical properties on cell behavior was assessed. Comprehensive analysis using scanning electron microscopy, real-time PCR, and endothelial cell viability assays revealed that cell-scaffold interactions are predominantly governed by the surface properties of the fibers. We established that the relative concentration of proteins (gelatin, albumin, and their mixtures) is a key factor controlling fiber surface modification and, consequently, cell-material interactions. This finding highlights the potential for optimizing scaffold properties by fine-tuning protein ratios to direct biological responses for specific clinical applications.