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<p>Copper nanoparticles (CuNPs) possess distinctive physicochemical properties with significant biomedical potential. In this study, copper glycinate nanoparticles (Cu(Gly)NPs) were synthesized via chemical reduction using ascorbic acid as a capping agent, and their structural, functional, and biological properties were systematically evaluated. UV–Vis spectroscopy revealed a characteristic absorption band at ~ 350&#xa0;nm, indicative of copper-based nanoparticle formation, while FTIR analysis revealed functional groups responsible for binding and stabilization. XRD analysis indicated a crystalline structure with an average particle size of 20–27&#xa0;nm, and SEM confirmed morphological variations influenced by synthesis parameters. Biologically, Cu(Gly)NPs exhibited dose-dependent antibacterial activity, with <i>Staphylococcus aureus</i> showing greater susceptibility than <i>Escherichia coli</i>. In vivo antioxidant studies demonstrated significant increases in SOD, CAT, and GPx activities in treated albino mice, suggesting potent oxidative stress protection. Cytotoxicity assessment revealed mild toxicity with an LC50 of 16.5 ppm. Molecular docking indicated moderate binding affinities with antioxidant enzymes (SOD: − 3.8&#xa0;kcal/mol) and stable interactions with the antibacterial protein 4QGG, supporting enhanced enzymatic activity and antibacterial potential. Overall, Cu(Gly)NPs displayed promising multifunctional bioactivities, integrating antibacterial, antioxidant, and cytotoxic properties, with strong evidence of therapeutic relevance. These findings highlight their potential for biomedical applications, warranting further optimization and preclinical evaluation.</p>

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Experimental and in Silico Investigation of Copper Glycinate Nanoparticles for Antibacterial, Antioxidant, and Cytotoxic Applications

  • 期刊论文

  • 发表日期：

    2026年2月4日

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  • Ali Umar,
  • Dibakar Roy,
  • Ali Abid,
  • Turaeva Gulnoz,
  • Khushnud Azizjanov,
  • Murodjon Yaxshimuratov,
  • Subhashree Roy,
  • Renu Sharma,
  • Misbah Ullah Khan

摘要

Copper nanoparticles (CuNPs) possess distinctive physicochemical properties with significant biomedical potential. In this study, copper glycinate nanoparticles (Cu(Gly)NPs) were synthesized via chemical reduction using ascorbic acid as a capping agent, and their structural, functional, and biological properties were systematically evaluated. UV–Vis spectroscopy revealed a characteristic absorption band at ~ 350 nm, indicative of copper-based nanoparticle formation, while FTIR analysis revealed functional groups responsible for binding and stabilization. XRD analysis indicated a crystalline structure with an average particle size of 20–27 nm, and SEM confirmed morphological variations influenced by synthesis parameters. Biologically, Cu(Gly)NPs exhibited dose-dependent antibacterial activity, with Staphylococcus aureus showing greater susceptibility than Escherichia coli. In vivo antioxidant studies demonstrated significant increases in SOD, CAT, and GPx activities in treated albino mice, suggesting potent oxidative stress protection. Cytotoxicity assessment revealed mild toxicity with an LC50 of 16.5 ppm. Molecular docking indicated moderate binding affinities with antioxidant enzymes (SOD: − 3.8 kcal/mol) and stable interactions with the antibacterial protein 4QGG, supporting enhanced enzymatic activity and antibacterial potential. Overall, Cu(Gly)NPs displayed promising multifunctional bioactivities, integrating antibacterial, antioxidant, and cytotoxic properties, with strong evidence of therapeutic relevance. These findings highlight their potential for biomedical applications, warranting further optimization and preclinical evaluation.