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<p>In this study, we have synthesized a bio-composite nanomaterial in a facile and economical one pot reaction for the detection of lead cations in water. The nanomaterial was fabricated with a 22-mer single stranded (ss)DNA oligonucleotide aptamer 5′-TTCTTTCTTCCCCTTGTTTGTT-3′ (PbApt), which served as the detection element. After determining the selectivity and sensitivity of the aptamer for lead cations against other competing heavy metal ions (copper, cadmium, and nickel), the aptamer was then immobilized covalently on APTES coated CuO nanoparticles via NHS-EDC crosslinking. The aptamer – nanoparticle conjugate was then annealed on a conductive ITO surface and served as the transducer element. The fully assembled aptameric nano-biosensor was characterized extensively using physicochemical techniques. The hybrid nanomaterial was analyzed for sensitivity and selectivity using cyclic voltammetry, where it was found to be selective for lead cations, having limit of detection of 159.4693 nM and 597.73 nM in ideal buffer and river water respectively. The significance of the study is that such facile, economic and specific nanosensors can be fabricated for several localized purposes including the onsite detection of pollutants.</p>

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Fabrication of an Aptasensor for specific, Quick and Easy Detection of Lead in Water: Physicochemical Studies

  • 期刊论文

  • 发表日期：

    2026年1月16日

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  • Niloy Sarkar,
  • Amit Singh,
  • Pankaj Kumar,
  • Kajal Jindal,
  • Arijit Chowdhuri,
  • Monika Tomar,
  • Radhey Shyam Sharma,
  • Mahima Kaushik

摘要

In this study, we have synthesized a bio-composite nanomaterial in a facile and economical one pot reaction for the detection of lead cations in water. The nanomaterial was fabricated with a 22-mer single stranded (ss)DNA oligonucleotide aptamer 5′-TTCTTTCTTCCCCTTGTTTGTT-3′ (PbApt), which served as the detection element. After determining the selectivity and sensitivity of the aptamer for lead cations against other competing heavy metal ions (copper, cadmium, and nickel), the aptamer was then immobilized covalently on APTES coated CuO nanoparticles via NHS-EDC crosslinking. The aptamer – nanoparticle conjugate was then annealed on a conductive ITO surface and served as the transducer element. The fully assembled aptameric nano-biosensor was characterized extensively using physicochemical techniques. The hybrid nanomaterial was analyzed for sensitivity and selectivity using cyclic voltammetry, where it was found to be selective for lead cations, having limit of detection of 159.4693 nM and 597.73 nM in ideal buffer and river water respectively. The significance of the study is that such facile, economic and specific nanosensors can be fabricated for several localized purposes including the onsite detection of pollutants.