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<p>This research presents an eco-friendly technique for synthesizing zinc oxide nanoparticles (ZnONPs) using peppermint (<i>Mentha piperita</i>) extract as both a reducing and stabilizing agent. The synthesis involved a controlled reaction between zinc nitrate hexahydrate [Zn(NO<sub>3</sub>)<sub>2</sub>.6H<sub>2</sub>O] and the Peppermint extract. The resulting ZnONPs were characterized using SEM, EDXS, TEM, XRD, FTIR, and UV–Vis spectroscopy. SEM revealed well-defined triangular crystals, while TEM showed spherical nanoparticles sized 20–50&#xa0;nm in diameter. EDXS confirmed the elemental composition of Zinc (80.4%), and Oxygen (19.6%). XRD analysis validated the crystalline structure, and FTIR identified functional groups associated with phenolics and proteins. UV–Vis spectra displayed an absorption peak at 210&#xa0;nm is likely due to residual phytochemicals in the peppermint extract, such as phenolic compounds and flavonoids. Antimicrobial testing using the well diffusion method showed inhibitory zones of 0.051&#xa0;mm, 0.12&#xa0;mm, and 0.25&#xa0;mm, respectively. at ZnONPs concentrations of 15, 25, and 35&#xa0;mg/mL, respectively. The results demonstrate effective bacterial inhibition zone (5&#xa0;mm), with the highest concentration observed at 35&#xa0;mg/mL concentration. This green synthesis method is sustainable and holds potential for applications in antibacterial technologies and various other industries. The ZnONPs demonstrated significant antibacterial properties, suggesting their potential for further research in biological and materials science.</p>

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Eco-friendly Zinc Oxide Nanoparticles from Peppermint: Synthesis, Characterization, and Antimicrobial Evaluation

  • 期刊论文

  • 发表日期：

    2026年1月16日

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  • Shahira Al-Risi,
  • Arshad Ali,
  • Maryam Al-Zadjali,
  • Ali Masoud Al-Subhi,
  • Abdullah M. Al-Sadi,
  • Muhammad Shafiq Shahid

摘要

This research presents an eco-friendly technique for synthesizing zinc oxide nanoparticles (ZnONPs) using peppermint (Mentha piperita) extract as both a reducing and stabilizing agent. The synthesis involved a controlled reaction between zinc nitrate hexahydrate [Zn(NO3)2.6H2O] and the Peppermint extract. The resulting ZnONPs were characterized using SEM, EDXS, TEM, XRD, FTIR, and UV–Vis spectroscopy. SEM revealed well-defined triangular crystals, while TEM showed spherical nanoparticles sized 20–50 nm in diameter. EDXS confirmed the elemental composition of Zinc (80.4%), and Oxygen (19.6%). XRD analysis validated the crystalline structure, and FTIR identified functional groups associated with phenolics and proteins. UV–Vis spectra displayed an absorption peak at 210 nm is likely due to residual phytochemicals in the peppermint extract, such as phenolic compounds and flavonoids. Antimicrobial testing using the well diffusion method showed inhibitory zones of 0.051 mm, 0.12 mm, and 0.25 mm, respectively. at ZnONPs concentrations of 15, 25, and 35 mg/mL, respectively. The results demonstrate effective bacterial inhibition zone (5 mm), with the highest concentration observed at 35 mg/mL concentration. This green synthesis method is sustainable and holds potential for applications in antibacterial technologies and various other industries. The ZnONPs demonstrated significant antibacterial properties, suggesting their potential for further research in biological and materials science.