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<p>Recently, wound healing has attracted the attention of researchers due to its importance in many chronic diseases such as diabetes. Here, we report a simple, novel, and effective aerosolized nanoparticulate structure for accelerating wound healing in a Wistar rat model via sustained delivery of neomycin and <i>Salvadora persica</i> (MISWAK) crude extract. The nanoparticulate structure comprises a core of ZnO surrounded by a double shell of alginate and chitosan. The core-double shell nanoparticles (CDS NPs) were formulated as an aerosol to facilitate the topical application of the nanoparticles onto the wound sites. The CDS NPs were characterized by electron microscopy, X-ray diffractometry, dynamic light scattering, and zeta potential measurements, as well as FTIR spectroscopy. The CDS NPs demonstrated an average particle size of ca. 53.5&#xa0;nm and a zeta potential of ca. -35 mV, indicating their high surface area-to-volume ratio and good colloidal stability. In-vitro MTT assay revealed the biocompatibility of the investigated nanostructure, and the inhibition disc method revealed their potent effect on both Gram-positive and Gram-negative bacteria. In addition, the in vitro scratching assay showed the ability of the CDS NPs to induce cell attachment and scratch covering using the HFB-4 (normal human melanocyte) cell line. Animal model experiments demonstrated that the CDS NPs may alleviate the inflammatory symptoms via increasing IL-10 and decreasing TNF-α levels. Moreover, histopathological examination of skin samples excised from animals treated with the CDS NPs depicted that the suggested system enhances wound healing via inducing epithelialization and collagen deposition in the wound area in addition to inhibiting the inflammatory response.</p> Graphical Abstract <p></p>

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Aerosolized Multifunctional Core-Double Shell ZnO-Alginate-Chitosan Nanoparticulate System for Wound Healing

  • 期刊论文

  • 发表日期：

    2025年12月17日

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  • Amira S. Mohamed,
  • Ahmed S. Abo Dena,
  • Mohamed A. Omar,
  • Gehad G. Mohamed,
  • Ibrahim M. El-Sherbiny

摘要

Recently, wound healing has attracted the attention of researchers due to its importance in many chronic diseases such as diabetes. Here, we report a simple, novel, and effective aerosolized nanoparticulate structure for accelerating wound healing in a Wistar rat model via sustained delivery of neomycin and Salvadora persica (MISWAK) crude extract. The nanoparticulate structure comprises a core of ZnO surrounded by a double shell of alginate and chitosan. The core-double shell nanoparticles (CDS NPs) were formulated as an aerosol to facilitate the topical application of the nanoparticles onto the wound sites. The CDS NPs were characterized by electron microscopy, X-ray diffractometry, dynamic light scattering, and zeta potential measurements, as well as FTIR spectroscopy. The CDS NPs demonstrated an average particle size of ca. 53.5 nm and a zeta potential of ca. -35 mV, indicating their high surface area-to-volume ratio and good colloidal stability. In-vitro MTT assay revealed the biocompatibility of the investigated nanostructure, and the inhibition disc method revealed their potent effect on both Gram-positive and Gram-negative bacteria. In addition, the in vitro scratching assay showed the ability of the CDS NPs to induce cell attachment and scratch covering using the HFB-4 (normal human melanocyte) cell line. Animal model experiments demonstrated that the CDS NPs may alleviate the inflammatory symptoms via increasing IL-10 and decreasing TNF-α levels. Moreover, histopathological examination of skin samples excised from animals treated with the CDS NPs depicted that the suggested system enhances wound healing via inducing epithelialization and collagen deposition in the wound area in addition to inhibiting the inflammatory response.

Graphical Abstract