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<p>In the past few years, there has been a significant focus on the utilization of copper oxide in biological applications due to its low toxicity and biocompatibility. In this context, Pure, Zn-doped, and Zn/Ni co-doped CuO nanostructures with large surface area were synthesized via the hydrothermal technique, employing varying Ni concentrations (1%, 2%, and 3%) along with a constant Zn concentration. The structural, morphological, and optical properties of the obtained samples were systematically investigated. The incorporation of Zn and Ni into copper oxide was found to have a notable influence on the crystallite size, lattice parameters, and band gap energies. XRD analysis confirmed a monoclinic CuO polycrystalline phase with a preferred orientation along the (− 111) plane across all samples. FE-SEM observations revealed nanoparticles with a cauliflower-shaped hierarchical morphology, consistent with the formation of CuO nanostructures as supported by XRD results. Optical measurements indicated band gap values ranging from 2.58 to 1.82 eV. The antimicrobial activity of pure CuO, Zn-doped CuO, and Zn/Ni co-doped CuO against S. aureus and E. coli strains was evaluated using inhibition zone assays. While all samples displayed significant antibacterial properties, the 3% Zn/3% Ni co-doped CuO exhibited the highest activity, showing a two-fold enhancement compared to pure. These findings suggest that the synthesized CuO nanostructures hold great promise as efficient antibacterial agents for biomedical and environmental applications.</p>

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Enhanced Antibacterial Activity of Zn/Ni co-doped Copper Oxide Cauliflowers Synthesized via Hydrothermal Technique

  • 期刊论文

  • 发表日期：

    2025年11月11日

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  • Ali Khudair Abbas,
  • Selma M. H. AL-Jawad,
  • Natheer Jamal Imran,
  • Hawraa H. Abbas,
  • Ghufran K. Ibadi

摘要

In the past few years, there has been a significant focus on the utilization of copper oxide in biological applications due to its low toxicity and biocompatibility. In this context, Pure, Zn-doped, and Zn/Ni co-doped CuO nanostructures with large surface area were synthesized via the hydrothermal technique, employing varying Ni concentrations (1%, 2%, and 3%) along with a constant Zn concentration. The structural, morphological, and optical properties of the obtained samples were systematically investigated. The incorporation of Zn and Ni into copper oxide was found to have a notable influence on the crystallite size, lattice parameters, and band gap energies. XRD analysis confirmed a monoclinic CuO polycrystalline phase with a preferred orientation along the (− 111) plane across all samples. FE-SEM observations revealed nanoparticles with a cauliflower-shaped hierarchical morphology, consistent with the formation of CuO nanostructures as supported by XRD results. Optical measurements indicated band gap values ranging from 2.58 to 1.82 eV. The antimicrobial activity of pure CuO, Zn-doped CuO, and Zn/Ni co-doped CuO against S. aureus and E. coli strains was evaluated using inhibition zone assays. While all samples displayed significant antibacterial properties, the 3% Zn/3% Ni co-doped CuO exhibited the highest activity, showing a two-fold enhancement compared to pure. These findings suggest that the synthesized CuO nanostructures hold great promise as efficient antibacterial agents for biomedical and environmental applications.