Scholarlink

<p>Oral cancer remains a significant global health burden, contributing to high mortality rates due to late diagnosis and limited therapeutic efficacy. In this study, we investigated the anticancer potential of a synthesized reduced graphene oxide–titanium dioxide (rGO–TiO₂) nanocomposite against the oral cancer KB cell line. The nanocomposite was extensively characterized using various analytical techniques. UV–Visible spectroscopy revealed a strong absorption peak at 351 nm. SEM analysis showed that the rGO–TiO₂ nanocomposite consists of rGO sheets with embedded spherical TiO₂ nanoparticles, averaging 96 ± 12&#xa0;nm in size. DLS analysis indicated a hydrodynamic diameter of 552.0&#xa0;nm with a PDI of 0.485, while zeta potential analysis revealed a surface charge of − 44.2 mV, suggesting good stability. To evaluate its anticancer efficacy, the MTT assay was used to assess cytotoxicity. Furthermore, AO/EtBr dual staining, DCF-DA assay, and Rhodamine 123 staining were employed to investigate apoptosis, ROS generation, and mitochondrial membrane potential (ΔΨm), respectively. Additionally, colony formation and cell migration assays demonstrated significant inhibition of cancer cell proliferation and migration. Biochemical assays revealed increased oxidative stress, as evidenced by elevated lipid peroxidation and altered activity of antioxidant enzymes. These findings suggest that the rGO–TiO₂ nanocomposite exerts its anticancer effects by inducing oxidative stress-mediated apoptosis and suppressing metastatic potential in oral cancer cells. This study highlights the rGO–TiO₂ nanocomposites as a potential therapeutic agent for oral cancer treatment.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Exploring the Anticancer Effect of Reduced Graphene Oxide–Titanium Dioxide (rGO-TiO₂) Nanocomposite against Oral Cancer

  • 期刊论文

  • 发表日期：

    2025年10月24日

查看全文
  • Mohanprasanth Aruchamy,
  • Ramanathan Snega,
  • Muthuvel Surya,
  • Saravanan Muthupandian

摘要

Oral cancer remains a significant global health burden, contributing to high mortality rates due to late diagnosis and limited therapeutic efficacy. In this study, we investigated the anticancer potential of a synthesized reduced graphene oxide–titanium dioxide (rGO–TiO₂) nanocomposite against the oral cancer KB cell line. The nanocomposite was extensively characterized using various analytical techniques. UV–Visible spectroscopy revealed a strong absorption peak at 351 nm. SEM analysis showed that the rGO–TiO₂ nanocomposite consists of rGO sheets with embedded spherical TiO₂ nanoparticles, averaging 96 ± 12 nm in size. DLS analysis indicated a hydrodynamic diameter of 552.0 nm with a PDI of 0.485, while zeta potential analysis revealed a surface charge of − 44.2 mV, suggesting good stability. To evaluate its anticancer efficacy, the MTT assay was used to assess cytotoxicity. Furthermore, AO/EtBr dual staining, DCF-DA assay, and Rhodamine 123 staining were employed to investigate apoptosis, ROS generation, and mitochondrial membrane potential (ΔΨm), respectively. Additionally, colony formation and cell migration assays demonstrated significant inhibition of cancer cell proliferation and migration. Biochemical assays revealed increased oxidative stress, as evidenced by elevated lipid peroxidation and altered activity of antioxidant enzymes. These findings suggest that the rGO–TiO₂ nanocomposite exerts its anticancer effects by inducing oxidative stress-mediated apoptosis and suppressing metastatic potential in oral cancer cells. This study highlights the rGO–TiO₂ nanocomposites as a potential therapeutic agent for oral cancer treatment.