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Abstract <p>The increasing demand for efficiently safer nanoparticles in biomedical applications has led to research into alternatives, as conventional silver nanoparticles (AgNPs) often exhibit noticeable cytotoxic effects. Nevertheless, green silver nanoparticles conjugated with peptides (GnanoAgPs) offer reduced cytotoxicity with more specific target. This study promptly pinpoints the synthesized AgNPs’ characteristics and its safest effects along with vitally required immunomodulatory and anti-inflammatory properties of GnanoAgPs in canine macrophage-like monocytes (MLMs) as a model system. Here using barberry leaf extract–based GnanoAg was synthesized, and then with cell penetrating peptide GnanoAgPs was eventually formulated. Physicochemical characteristics of the yielded nanoparticles (NPs) were evaluated with UV–Vis spectrophotometry, scanning electron microscopy (SEM), dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FTIR). In addition, GnanoAgP-exposed MLM quality/viability and mRNA expression of some key immune-and-inflammatory-related molecules were examined with flow cytometry and qPCR, respectively. We found that 0.1&#xa0;μg GnanoAgPs/ml resulted in an immunomodulatory and anti-inflammatory microenvironment of MLMs with remarkable decrease of NRLP3, IL-6, C-type lectin, and growth arrest–specific protein (GAS) 6 along with upregulation on TLR2, IL-1β, BCL2, and caspase (Cas) 9 and unchanged TLR4 and Cas 3. Overall, the study sheds lights on the notion that our synthesized GnanoAgPs could potentially act as anti-inflammatory and pro-apoptotic biomaterial and effective therapeutic agents in mitigating inflammation without significant cytotoxicity, which can be used in veterinary-human and-biomedicine.</p> Graphical Abstract <p>Therapeutically relevant and more functionalized green silver nanoparticles (GnanoAg)&#xa0;conjugated with MiRGD peptides (GnanoAgPs) were synthesized with physicochemically&#xa0;acceptable nanoparticles. Their immunobiological/molecular impacts reveal GnanoAgPs’&#xa0;relatively safe anti-inflammatory properties in companion animals and human.</p> <p></p>

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Synthesized Cell Penetrating Peptide-Functionalized Green Nanosilver Promisingly Exhibits Anti-inflammatory Properties in Cultured Canine Macrophage-Like Monocytes

  • 期刊论文

  • 发表日期：

    2025年12月17日

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  • Fatemeh Ayazi Roozbahani,
  • Bita Fazel,
  • Sepideh Moradkhani,
  • Saman Hosseinkhani,
  • Jalil Mehrzad

摘要

Abstract

The increasing demand for efficiently safer nanoparticles in biomedical applications has led to research into alternatives, as conventional silver nanoparticles (AgNPs) often exhibit noticeable cytotoxic effects. Nevertheless, green silver nanoparticles conjugated with peptides (GnanoAgPs) offer reduced cytotoxicity with more specific target. This study promptly pinpoints the synthesized AgNPs’ characteristics and its safest effects along with vitally required immunomodulatory and anti-inflammatory properties of GnanoAgPs in canine macrophage-like monocytes (MLMs) as a model system. Here using barberry leaf extract–based GnanoAg was synthesized, and then with cell penetrating peptide GnanoAgPs was eventually formulated. Physicochemical characteristics of the yielded nanoparticles (NPs) were evaluated with UV–Vis spectrophotometry, scanning electron microscopy (SEM), dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FTIR). In addition, GnanoAgP-exposed MLM quality/viability and mRNA expression of some key immune-and-inflammatory-related molecules were examined with flow cytometry and qPCR, respectively. We found that 0.1 μg GnanoAgPs/ml resulted in an immunomodulatory and anti-inflammatory microenvironment of MLMs with remarkable decrease of NRLP3, IL-6, C-type lectin, and growth arrest–specific protein (GAS) 6 along with upregulation on TLR2, IL-1β, BCL2, and caspase (Cas) 9 and unchanged TLR4 and Cas 3. Overall, the study sheds lights on the notion that our synthesized GnanoAgPs could potentially act as anti-inflammatory and pro-apoptotic biomaterial and effective therapeutic agents in mitigating inflammation without significant cytotoxicity, which can be used in veterinary-human and-biomedicine.

Graphical Abstract

Therapeutically relevant and more functionalized green silver nanoparticles (GnanoAg) conjugated with MiRGD peptides (GnanoAgPs) were synthesized with physicochemically acceptable nanoparticles. Their immunobiological/molecular impacts reveal GnanoAgPs’ relatively safe anti-inflammatory properties in companion animals and human.