Scholarlink

<p>Orthodontic treatments are often associated with enamel demineralization and white spot lesions, which can lead to various processes. To address these issues, innovative materials are being explored for their antibacterial properties. This study evaluates the efficacy of newly formed orthodontic polyether ether ketone (PEEK) beads coated with hydroxyapatite nanoparticles conjugated with the GL13K-hydroxyapatite (HAP) prepared via chemical and laser methods. GL13K-HAP nanocomposites were synthesized using chemical and eco-friendly laser ablation techniques and characterized using ultraviolet-visible (UV-visible) spectroscopy, Fourier-transform infrared (FTIR), Raman spectroscopy, transmission electron microscopy (TEM), and field emission scanning electron microscopy (FESEM). The PEEK beads were coated with these nanocomposites, and their antibacterial efficacy against various bacterial strains and cytotoxicity on REF cells were assessed. Cytotoxicity was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the bactericidal effect was evaluated through the agar well diffusion assay and acridine orange/ethidium bromide (AO/EtBr) staining. The nanocomposites exhibited distinct morphological and optical properties. Coated PEEK beads prepared by both methods demonstrated significant antibacterial activity, with zones of inhibition ranging from 9 to 22&#xa0;mm against bacterial strains. Cytotoxicity assays revealed biocompatibility of the coated beads, with no significant reduction in REF cell viability, highlighting their potential for clinical use. The GL13K-HAP nanocomposite coating enhanced the antibacterial properties of orthodontic PEEK beads without compromising biocompatibility, making them a promising material for reducing bacterial adhesion and preventing enamel demineralization by simply including these beads throughout all stages of fixed orthodontic treatments.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Antibacterial Effect of Novel Orthodontic PEEK Beads Coated with a Conjugate of Fish Scale Hydroxy-Apatite Nanoparticles and GL13K Peptide

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年9月30日

查看全文
  • Harraa S. Mohammed-Salih,
  • Rana I. Mahmood,
  • Ataa Ghazi,
  • Ali Fadhil AlQrimli,
  • Taha M. Rashid,
  • Jameel R. Al-Obaidi,
  • Faridah Lisa Supian,
  • Majid S. Jabir

摘要

Orthodontic treatments are often associated with enamel demineralization and white spot lesions, which can lead to various processes. To address these issues, innovative materials are being explored for their antibacterial properties. This study evaluates the efficacy of newly formed orthodontic polyether ether ketone (PEEK) beads coated with hydroxyapatite nanoparticles conjugated with the GL13K-hydroxyapatite (HAP) prepared via chemical and laser methods. GL13K-HAP nanocomposites were synthesized using chemical and eco-friendly laser ablation techniques and characterized using ultraviolet-visible (UV-visible) spectroscopy, Fourier-transform infrared (FTIR), Raman spectroscopy, transmission electron microscopy (TEM), and field emission scanning electron microscopy (FESEM). The PEEK beads were coated with these nanocomposites, and their antibacterial efficacy against various bacterial strains and cytotoxicity on REF cells were assessed. Cytotoxicity was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the bactericidal effect was evaluated through the agar well diffusion assay and acridine orange/ethidium bromide (AO/EtBr) staining. The nanocomposites exhibited distinct morphological and optical properties. Coated PEEK beads prepared by both methods demonstrated significant antibacterial activity, with zones of inhibition ranging from 9 to 22 mm against bacterial strains. Cytotoxicity assays revealed biocompatibility of the coated beads, with no significant reduction in REF cell viability, highlighting their potential for clinical use. The GL13K-HAP nanocomposite coating enhanced the antibacterial properties of orthodontic PEEK beads without compromising biocompatibility, making them a promising material for reducing bacterial adhesion and preventing enamel demineralization by simply including these beads throughout all stages of fixed orthodontic treatments.