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<p>The present study focuses on the development and evaluation of hydrogels based on Pluronic F127 and polyvinyl alcohol (PVA) for controlled drug delivery applications for curcumin. The curcumin-loaded F127/PVA hydrogels were synthesized using a freeze–thaw method and optimized based on drug content (%DL ranging from 64.20 ± 0.8 to 95.80 ± 0.4%) and encapsulation efficiency (%EE ranging from 98 ± 0.7 to 99.80 ± 0.1%), physical integrity, in vitro drug release, and cytotoxicity profiles. Structural and physicochemical properties of the synthesized hydrogels were characterized using UV–visible spectroscopy, rheology, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) techniques, confirming the successful loading of curcumin into polymer matrices and showing the hydrogel morphology and crystallinity. Antibacterial studies of the hydrogel clearly showed significant inhibition against <i>S. aureus</i> and <i>E. coli</i>, proving its potent antimicrobial activity: PF0.5C reduced <i>E. coli</i> by 65.02% and <i>S. aureus</i> by 6.01%; PF1C reduced <i>E. coli</i> by 76.09% and <i>S. aureus</i> by 80.74%; PF1.5C and PF2C showed highly potent Antibacterial action with 99.82% And 99.89% reduction of <i>E. coli</i> And 99.99% reduction of <i>S. aureus</i>. Cytotoxicity assessment using the MTT assay indicated that the hydrogel was biocompatible with normal fibroblast cells while exhibiting dose-dependent anticancer activity against A549 lung cancer cells with an IC₅₀ of 41.2 ± 2.1&#xa0;µg/mL. AO/EB staining further confirmed apoptosis induction in cancer cells. Here, to elucidate the molecular interactions between the drug and gel components, density functional theory (DFT) calculations and molecular docking studies were conducted, supporting the mechanism of action at the molecular level. Overall, the present work demonstrates that Pluronic F127/PVA hydrogel systems offer a promising platform for the controlled delivery of highly hydrophobic drugs like curcumin.</p> Graphical Abstract <p></p>

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Development and Evaluation of Curcumin-Loaded Pluronic-Polyvinyl Alcohol hydrogel Systems: Synthesis, Characterization, Biological and Computational Investigations

  • 期刊论文

  • 发表日期：

    2025年9月29日

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  • Hemil S. Patel,
  • Ishita Saxena,
  • Poonam N. Bhargriya,
  • Bhavesh B. Makwana,
  • Heena A. Parmar,
  • Rakesh K. Sharma

摘要

The present study focuses on the development and evaluation of hydrogels based on Pluronic F127 and polyvinyl alcohol (PVA) for controlled drug delivery applications for curcumin. The curcumin-loaded F127/PVA hydrogels were synthesized using a freeze–thaw method and optimized based on drug content (%DL ranging from 64.20 ± 0.8 to 95.80 ± 0.4%) and encapsulation efficiency (%EE ranging from 98 ± 0.7 to 99.80 ± 0.1%), physical integrity, in vitro drug release, and cytotoxicity profiles. Structural and physicochemical properties of the synthesized hydrogels were characterized using UV–visible spectroscopy, rheology, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) techniques, confirming the successful loading of curcumin into polymer matrices and showing the hydrogel morphology and crystallinity. Antibacterial studies of the hydrogel clearly showed significant inhibition against S. aureus and E. coli, proving its potent antimicrobial activity: PF0.5C reduced E. coli by 65.02% and S. aureus by 6.01%; PF1C reduced E. coli by 76.09% and S. aureus by 80.74%; PF1.5C and PF2C showed highly potent Antibacterial action with 99.82% And 99.89% reduction of E. coli And 99.99% reduction of S. aureus. Cytotoxicity assessment using the MTT assay indicated that the hydrogel was biocompatible with normal fibroblast cells while exhibiting dose-dependent anticancer activity against A549 lung cancer cells with an IC₅₀ of 41.2 ± 2.1 µg/mL. AO/EB staining further confirmed apoptosis induction in cancer cells. Here, to elucidate the molecular interactions between the drug and gel components, density functional theory (DFT) calculations and molecular docking studies were conducted, supporting the mechanism of action at the molecular level. Overall, the present work demonstrates that Pluronic F127/PVA hydrogel systems offer a promising platform for the controlled delivery of highly hydrophobic drugs like curcumin.

Graphical Abstract