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<p>Antimicrobial resistance is a silent, looming pandemic advancing toward a global crisis. In a quest for an alternative to antibiotics, the present study delves into the green synthesis of bioinspired phytobacteriogenic silver nanoparticles (pbAgNP) employing a cocktail of cell-free supernatants of <i>Ligilactobacillus salivarium</i>, <i>Pediococcus pentosaceus</i>, and <i>Moringa oleifera</i> leaf extract as a holistic and cost-effective approach with potential bioactivities. This study aimed to assess the physicochemical properties, in vitro stability, safety, antibiofouling, antioxidant, and antibacterial efficacy of pbAgNPs against multidrug-resistant <i>Staphylococcus aureus</i> strains. The colour change, surface plasma resonance (SPR), and powder X-ray diffraction (PXRD) analysis confirmed the formation and crystalline nature of the pbAgNPs. The Fourier transform infrared spectroscopy (FTIR) analysis displayed absorbance bands corresponding to potential functional groups as capping and stabilising agents. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images displayed a quasi-spherical shape of the nanoparticles. The antibacterial potency of pbAgNP was evaluated by appraising the minimum inhibitory concentration (0.010&#xa0;mg/mL) and minimum bactericidal concentration (0.040&#xa0;mg/mL) against MDR <i>S. aureus.</i> Furthermore, pbAgNP exhibited complete inhibition of MDR-<i>S. aureus</i> strains in a time- and dose-dependent manner and exhibited significant antibiofilm and antioxidant potential. Besides addressing the antimicrobial resistance, the pbAgNPs demonstrated exceptional versatility by maintaining physicochemical stability under elevated temperatures, varying pH levels, exposure to cationic salts, proteinase K, and chicken sera. The biocompatibility of pbAgNP was confirmed through cytotoxicity and haemolysis assays on HT-29 cell lines, chicken erythrocytes, and gut lactobacilli. In conclusion, the applicability of pbAgNPs presents a benchmark for an eco-friendly, sustainable alternative to traditional antibiotics, with enhanced antimicrobial properties, biocompatibility extending their application in the biomedical landscape and food industry, especially in the poultry feed industry as an antibiotic residue-free scalable postbiotic nanoformulation, aligning with the one health framework.</p>

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Unravelling the Synthesis, Characterisation, and Bioactivities of Novel Biomimetic Phytobacteriogenic Nanosilver Particles Against Multidrug-Resistant Staphylococcus aureus: A Sustainable One Health Approach

  • 期刊论文

  • 发表日期：

    2025年10月28日

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  • Amruta Nair,
  • Varsha Unni,
  • Florence Evangeline,
  • Sukhadeo Barbuddhe

摘要

Antimicrobial resistance is a silent, looming pandemic advancing toward a global crisis. In a quest for an alternative to antibiotics, the present study delves into the green synthesis of bioinspired phytobacteriogenic silver nanoparticles (pbAgNP) employing a cocktail of cell-free supernatants of Ligilactobacillus salivarium, Pediococcus pentosaceus, and Moringa oleifera leaf extract as a holistic and cost-effective approach with potential bioactivities. This study aimed to assess the physicochemical properties, in vitro stability, safety, antibiofouling, antioxidant, and antibacterial efficacy of pbAgNPs against multidrug-resistant Staphylococcus aureus strains. The colour change, surface plasma resonance (SPR), and powder X-ray diffraction (PXRD) analysis confirmed the formation and crystalline nature of the pbAgNPs. The Fourier transform infrared spectroscopy (FTIR) analysis displayed absorbance bands corresponding to potential functional groups as capping and stabilising agents. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images displayed a quasi-spherical shape of the nanoparticles. The antibacterial potency of pbAgNP was evaluated by appraising the minimum inhibitory concentration (0.010 mg/mL) and minimum bactericidal concentration (0.040 mg/mL) against MDR S. aureus. Furthermore, pbAgNP exhibited complete inhibition of MDR-S. aureus strains in a time- and dose-dependent manner and exhibited significant antibiofilm and antioxidant potential. Besides addressing the antimicrobial resistance, the pbAgNPs demonstrated exceptional versatility by maintaining physicochemical stability under elevated temperatures, varying pH levels, exposure to cationic salts, proteinase K, and chicken sera. The biocompatibility of pbAgNP was confirmed through cytotoxicity and haemolysis assays on HT-29 cell lines, chicken erythrocytes, and gut lactobacilli. In conclusion, the applicability of pbAgNPs presents a benchmark for an eco-friendly, sustainable alternative to traditional antibiotics, with enhanced antimicrobial properties, biocompatibility extending their application in the biomedical landscape and food industry, especially in the poultry feed industry as an antibiotic residue-free scalable postbiotic nanoformulation, aligning with the one health framework.