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<p>The present study investigates the biosynthesis of selenium nanoparticles (SeNPs) using the marine bacterial isolate <i>Stutzeromonas</i> sp. (MSB9). Characterisation of the SeNPs revealed a UV–Vis absorption peak at 265&#xa0;nm. FTIR analysis showed the active biomolecules involved in the capping and stabilisation of SeNPs. XRD analysis evidenced the crystalline structure of SeNPs, while FE-SEM confirmed spherical morphology with an average size of 85.2 ± 29.9&#xa0;nm. EDX detected absorption peaks corresponding to elemental selenium. The SeNPs exhibited broad-spectrum antibacterial activity (zones of inhibition: 16 to 18&#xa0;mm, MIC: 16&#xa0;µg/ml, MBC: 32&#xa0;µg/ml), particularly against <i>A. baumannii</i>. Biofilm formation of <i>A. baumannii</i> was significantly inhibited by the SeNPs, with an MBIC of 125&#xa0;μg/ml, as confirmed by microscopic imaging. A time-kill assay revealed that the SeNPs exert bacteriostatic effect at 1 × MIC and bactericidal effect at 4 × MIC. MATH assay showed that SeNPs at 256&#xa0;µg/ml reduced cell surface hydrophobicity (&lt; 30%) and inhibited EPS production (95%). The cytotoxicity assay using oral KB carcinoma cells revealed moderate cytotoxicity, with an IC<sub>50</sub> of 86.4&#xa0;µg/ml. These findings emphasise that <i>Stutzerimonas</i> sp.-derived SeNPs could be utilised as nanoantibiotics against medically important biofilm-associated bacterial infections and exhibit cytotoxicity against oral KB cells.</p>

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Biosynthesis of Selenium Nanoparticles from Marine Stutzerimonas sp. and Their Biomedical Implications

  • 期刊论文

  • 发表日期：

    2025年9月27日

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  • Ashwin SP,
  • Khushali K. Shah,
  • Dhanraj G,
  • Ramasubburayan R,
  • Bharathi S

摘要

The present study investigates the biosynthesis of selenium nanoparticles (SeNPs) using the marine bacterial isolate Stutzeromonas sp. (MSB9). Characterisation of the SeNPs revealed a UV–Vis absorption peak at 265 nm. FTIR analysis showed the active biomolecules involved in the capping and stabilisation of SeNPs. XRD analysis evidenced the crystalline structure of SeNPs, while FE-SEM confirmed spherical morphology with an average size of 85.2 ± 29.9 nm. EDX detected absorption peaks corresponding to elemental selenium. The SeNPs exhibited broad-spectrum antibacterial activity (zones of inhibition: 16 to 18 mm, MIC: 16 µg/ml, MBC: 32 µg/ml), particularly against A. baumannii. Biofilm formation of A. baumannii was significantly inhibited by the SeNPs, with an MBIC of 125 μg/ml, as confirmed by microscopic imaging. A time-kill assay revealed that the SeNPs exert bacteriostatic effect at 1 × MIC and bactericidal effect at 4 × MIC. MATH assay showed that SeNPs at 256 µg/ml reduced cell surface hydrophobicity (< 30%) and inhibited EPS production (95%). The cytotoxicity assay using oral KB carcinoma cells revealed moderate cytotoxicity, with an IC50 of 86.4 µg/ml. These findings emphasise that Stutzerimonas sp.-derived SeNPs could be utilised as nanoantibiotics against medically important biofilm-associated bacterial infections and exhibit cytotoxicity against oral KB cells.