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<p>Green and efficient biological silver nanoparticles (AgNPs) synthesis has garnered widespread interest. Protein-mediated silver nanoparticles synthesis presents a novel realm within biogenic methods. In early laboratory stages, protein SNA15 was successfully isolated from a microorganism known for efficient silver nanoparticles synthesis. To uncover the synthesis mechanism, a hypothesis of silver ion reduction through negatively charged amino acids on synthesized silver nanoparticles proteins was formulated. Through site-directed mutagenesis, protein surface charge properties were optimized, yielding recombinant protein TNA15. Notably, TNA15 exhibited an 8.08% enhancement in silver nanoparticles synthesis compared to its precursor. Synthesized silver nanoparticles IC50 values against <i>S. aureus</i>, <i>B. cereus</i>, <i>B. subtilis</i>, and <i>E. coli</i> were reduced from 130 μg /mL, 145.3 μg /mL, 123.6 μg/mL, and 122.9 μg/mL to 5.774 μg/mL, 5.534 μg/mL, 5.324 μg/mL, and 6.454 μg/mL. Experimental outcomes elucidate protein-mediated silver nanoparticles synthesis mechanism, highlighting the substantial enhancement achieved through site-directed mutagenesis.</p>

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Site-Directed Mutagenesis to Improve the Efficiency and Antimicrobial Effect of Protein for Silver Nanoparticles Synthesis

  • 期刊论文

  • 发表日期：

    2025年6月10日

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  • Yifei Chen,
  • Tong Chai,
  • Tianrui Wang,
  • Hangfei Xie,
  • Qianyun Wang,
  • Fuwei Yan,
  • Shengtao Zhang,
  • Xiangqian Li

摘要

Green and efficient biological silver nanoparticles (AgNPs) synthesis has garnered widespread interest. Protein-mediated silver nanoparticles synthesis presents a novel realm within biogenic methods. In early laboratory stages, protein SNA15 was successfully isolated from a microorganism known for efficient silver nanoparticles synthesis. To uncover the synthesis mechanism, a hypothesis of silver ion reduction through negatively charged amino acids on synthesized silver nanoparticles proteins was formulated. Through site-directed mutagenesis, protein surface charge properties were optimized, yielding recombinant protein TNA15. Notably, TNA15 exhibited an 8.08% enhancement in silver nanoparticles synthesis compared to its precursor. Synthesized silver nanoparticles IC50 values against S. aureus, B. cereus, B. subtilis, and E. coli were reduced from 130 μg /mL, 145.3 μg /mL, 123.6 μg/mL, and 122.9 μg/mL to 5.774 μg/mL, 5.534 μg/mL, 5.324 μg/mL, and 6.454 μg/mL. Experimental outcomes elucidate protein-mediated silver nanoparticles synthesis mechanism, highlighting the substantial enhancement achieved through site-directed mutagenesis.