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<p>Nano-carbon quantum dots (nano-CQDs) were prepared by a hydrothermal method using chitosan and melamine and characterized by FTIR, TEM, and SEM. The maximum excitation and emission wavelengths of the carbon dots were determined to be 338&#xa0;nm and 412&#xa0;nm, respectively, indicating their strong fluorescence. The fluorescence properties of complexes of nano-CQDs with dsDNA and bovine serum albumin (BSA) were investigated. It was shown that DNA and albumin acquire fluorescent properties upon complexation with CQDs. DNA and albumin labelled with CQDs exhibit electrochemical properties comparable to unlabeled biomolecules. DNA and DNA/CQD complexes showed well-separated irreversible waves with peak potentials corresponding to guanine, adenine, and thymine bases. BSA and BSA/CQD complexes exhibited one broad peak corresponding to electrochemical oxidation of tyrosine amino acids of the polypeptide backbone. This indicates the biocompatibility of CQDs and the preservation of the spatial structures of DNA and bovine serum albumin during the formation of fluorogenic complexes. The studied electrochemical behavior of nucleic acid or protein complexes with CQDs allows the use of these structures for the visualization of biological objects and their simultaneous electrochemical analysis.</p> Graphical Abstract <p></p>

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Carbon Quantum Dots in the Electroanalysis of DNA and Albumin

  • 期刊论文

  • 发表日期：

    2025年5月21日

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  • Lyubov E. Agafonova,
  • Tatiana V. Bulko,
  • Veronica V. Pronina,
  • Alexey V. Kuzikov,
  • Farida R. Allambergenova,
  • Nigora H. Qutlimurotova,
  • Victoria V. Shumyantseva

摘要

Nano-carbon quantum dots (nano-CQDs) were prepared by a hydrothermal method using chitosan and melamine and characterized by FTIR, TEM, and SEM. The maximum excitation and emission wavelengths of the carbon dots were determined to be 338 nm and 412 nm, respectively, indicating their strong fluorescence. The fluorescence properties of complexes of nano-CQDs with dsDNA and bovine serum albumin (BSA) were investigated. It was shown that DNA and albumin acquire fluorescent properties upon complexation with CQDs. DNA and albumin labelled with CQDs exhibit electrochemical properties comparable to unlabeled biomolecules. DNA and DNA/CQD complexes showed well-separated irreversible waves with peak potentials corresponding to guanine, adenine, and thymine bases. BSA and BSA/CQD complexes exhibited one broad peak corresponding to electrochemical oxidation of tyrosine amino acids of the polypeptide backbone. This indicates the biocompatibility of CQDs and the preservation of the spatial structures of DNA and bovine serum albumin during the formation of fluorogenic complexes. The studied electrochemical behavior of nucleic acid or protein complexes with CQDs allows the use of these structures for the visualization of biological objects and their simultaneous electrochemical analysis.

Graphical Abstract