Scholarlink

<p>This study formulates a stable oil-in-water (O/W) <i>Eucalyptus globulus</i> essential oil (EGEO) based nanoemulsion using low-energy spontaneous emulsification method&#xa0;with 15% oil content and 5% surfactant for studying its bioefficacy against <i>Spodoptera litura,</i> an economically important agricultural crop pest. The GC–MS study of <i>Eucalyptus</i> EO revealed 1,8-cineole (67.14%), α-pinene (7.50%), and α-terpineol (7.08%) as the major constituents. After optimizing the nanoemulsions using Span 80 and Tween 80, mean hydrodynamic diameter, polydispersity index (PDI), morphology, and physical stability were investigated. Results of transmission electron microscopy (TEM) and dynamic light scattering (DLS) measurements indicated nearly monodispersed formulations (PDI &lt; 0.3) with Tween 80 and polydispersed formulations with Span 80. Furthermore, long-term stabilities of nano-emulsions with each surfactant were assessed by measuring the droplet diameter for 50&#xa0;days. The nanoemulsions demonstrated promising repellency, ovicidal, and larvicidal activity against <i>S. litura</i>. A significant dose-dependent effect was observed on survival of <i>S. litura</i> with different concentrations of EGEO-based nanoemulsion under standard laboratory conditions. Ovicidal activity (LC<sub>50</sub> = 22.331&#xa0;mg&#xa0;mL<sup>−1</sup>) and egg hatchability were significantly affected by the nanoemulsions. In glass tunnel olfactometer tests, the nanoemulsion exhibited significant repellency towards neonate <i>S. litura</i> larvae. Molecular docking studies were performed using seven major components of EGEO based on GC–MS data against <i>S. litura</i> Chemosensory proteins (CSPs), which showed good binding affinity scores (− 4.0 to − 5.2&#xa0;kcal/mol) indicating synergic effect for activity of <i>E. globulus</i> EO. Thus, EGEO-based nanoemulsions may be a green biopesticide for sustainable management of <i>S. litura</i> to increase agricultural productivity<i>.</i></p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Eucalyptus globulus Essential Oil-Based Nano-emulsions: Comprehensive Development, In Vitro and In Silico Assessment Against Spodoptera litura (Fab.) (Lepidoptera: Noctuidae)

  • 期刊论文

  • 发表日期：

    2025年4月10日

查看全文
  • Ankur,
  • Nida,
  • Narender Singh,
  • Sanjiv Mullick,
  • Alka Gupta

摘要

This study formulates a stable oil-in-water (O/W) Eucalyptus globulus essential oil (EGEO) based nanoemulsion using low-energy spontaneous emulsification method with 15% oil content and 5% surfactant for studying its bioefficacy against Spodoptera litura, an economically important agricultural crop pest. The GC–MS study of Eucalyptus EO revealed 1,8-cineole (67.14%), α-pinene (7.50%), and α-terpineol (7.08%) as the major constituents. After optimizing the nanoemulsions using Span 80 and Tween 80, mean hydrodynamic diameter, polydispersity index (PDI), morphology, and physical stability were investigated. Results of transmission electron microscopy (TEM) and dynamic light scattering (DLS) measurements indicated nearly monodispersed formulations (PDI < 0.3) with Tween 80 and polydispersed formulations with Span 80. Furthermore, long-term stabilities of nano-emulsions with each surfactant were assessed by measuring the droplet diameter for 50 days. The nanoemulsions demonstrated promising repellency, ovicidal, and larvicidal activity against S. litura. A significant dose-dependent effect was observed on survival of S. litura with different concentrations of EGEO-based nanoemulsion under standard laboratory conditions. Ovicidal activity (LC50 = 22.331 mg mL−1) and egg hatchability were significantly affected by the nanoemulsions. In glass tunnel olfactometer tests, the nanoemulsion exhibited significant repellency towards neonate S. litura larvae. Molecular docking studies were performed using seven major components of EGEO based on GC–MS data against S. litura Chemosensory proteins (CSPs), which showed good binding affinity scores (− 4.0 to − 5.2 kcal/mol) indicating synergic effect for activity of E. globulus EO. Thus, EGEO-based nanoemulsions may be a green biopesticide for sustainable management of S. litura to increase agricultural productivity.

Graphical Abstract